白细胞介素6对雄激素活性的影响及在多囊卵巢综合征发病机制中的作用  

Effect of IL-6 on androgen activity and its role in the polycystic ovary syndrome pathogenesis

作　　者：洪岭[1] 商宏恺[2] 滕晓明[1] 李昆明[1] 杨阳[2] 张燕[2] 龚瑶[2] 童晓文[2] 

机构地区：[1]同济大学附属第一妇婴保健院生殖医学中心,上海200040 [2]同济大学附属同济医院妇产科,上海200065

出　　处：《中国实用妇科与产科杂志》2010年第3期213-215,共3页Chinese Journal of Practical Gynecology and Obstetrics

摘　　要：目的探讨白细胞介素6(IL-6)对雄激素活性的影响及在多囊卵巢综合征(PCOS)发病机制中的作用。方法在体外培养的猪卵泡内膜细胞和颗粒细胞培养液中分别加入IL-6(10ng/L、100ng/L、1000ng/L),观察IL-6对卵泡内膜细胞睾酮分泌及卵巢颗粒细胞雄激素受体(AR)mRNA表达的影响。结果在24h、48h、72h三个时间点分别与空白对照组(0ng/L)相比,加入IL-6后卵泡内膜细胞睾酮分泌无明显变化;在72h时通过Real-timePCR检测发现,IL-6(100ng/L、1000ng/L)对卵巢颗粒细胞AR-mRNA的表达具有上调作用。结论IL-6虽然没有直接促进卵巢睾酮分泌的作用,但却可以上调卵巢组织AR-mRNA的表达,间接增强雄激素活性,此可能与慢性亚临床炎症可引发PCOS的机制有关。Objective To study the effect of IL-6 on the secretion of testosterone in theca interna cells （TI） and on the expression of androgen receptor （AR） in ovarian granular cells（GC） and to explore the role of interleukin 6 （IL-6） in the polycystic ovary syndrome pathogenesis. Methods Different concentrations of IL-6（10ng/L、100ng/L、1000ng/L） were administered into cells medium of TI and GC. Secretion of testosterone in TI and expression of androgen receptor （AR） mRNA in GC were detected. Results The testosterone levels have no difference between the study groups （10ng/L,100ng/L,1000ng/L）and the control group（0ng/L）at 24h,48h,72h in the TI. The mRNA expressions of AR in GC were upregulated in groups with 100ng/L and 1000ng/L IL-6 at 72h by RT-PCR. Conclusion Although IL-6 can＇t induce testosterone secretion directly, but it can upregulate the mRNA expression of AR, so IL-6 can improve androgen activity indirectly, which probably can explain the phenomenon： chronic subclinical inflammation may result in PCOS.

关 键 词：白细胞介素6 多囊卵巢综合征 睾酮 雄激素受体 

分 类 号：R71[医药卫生—妇产科学]