中国北方沿海6个栉孔扇贝群体的AFLP分析  

AFLP Analysis on Six Populations of Zhikong Scallop (Chlamys farreri) Along the Northern Coastline of China

作　　者：李宁[1] 程洁[1] 孙鲁阳[1] 赵海波[1] 侯睿[1] 胡景杰[1] 包振民[1] 

机构地区：[1]中国海洋大学海洋生物遗传育种教育部重点实验室,海洋生命学院,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2010年第4期38-42,共5页Periodical of Ocean University of China

基　　金：国家高技术研究发展计划重大项目(2006AA10A408)资助

摘　　要：利用4对AFLP引物首次对我国北方沿海主要的6个栉孔扇贝养殖区的自然采苗群体进行了遗传多样性分析,共扩增得到191条清晰带谱,其中175条具有多态性。对各群体进行遗传多样性分析,结果表明各群体均具有较高的遗传多样性水平,香农式多样性指数在0.347 4～0.391 7之间。AMOVA结果表明遗传变异主要来源于群体内,占总遗传变异的87.22%。个体聚类结果表明,烟台、长岛、大连3个群体的个体聚在一起,可看做1个群体;而荣成群体的个体单独聚在一起,具有独立的遗传结构;胶南和日照的一部分个体聚在一起,其他大部分各群体单独聚在一起;推测是海洋环境和人工养殖影响的共同结果。The AFLP technique was used to analyze the genetic diversity of 6 natural populations of Zhikong scallop（Chlamys farreri） from the main cultured zones along the northern coastline of China.Based on 4 pairs of primers,191 clear and repeated DNA fragments were generated,of which 175 were polymorphic.The genetic diversity analysis of the populations showed high levels of genetic diversity of 6 populations,and the Shannon s genetic diversity index varied from 0.347 4 to 0.391 7.The AMOVA（an-alyzing molecular varaiance） analysis indicated 87.22% viaration were within populations.The phylogeny tree showed that the individuals of Rongcheng clustered together,and had formed its own dependent ge-netic structure.The Dalian,Changdao and Yantai populations formed a cluster that could be regarded as one population.Most of the individuals of Jiaonan and Rizhao populations clustered respectively,but a few individuals clustered together.The structure of the populations of Zhikong scallop along the nortern coastline of China may have resulted from the oceanic environment and the interference of artificial cul-ture.

关 键 词：栉孔扇贝(Chlamys farreri) 自然苗群体 养殖区 AFLP 遗传多样性 

分 类 号：S917[农业科学—水产科学]