Tim-3在小鼠心脏移植受者体内不同部位T淋巴细胞上表达的变化  

Changes of Tim - 3 expression in T lymphocytes from different sites in mice heart - transplant recipients

作　　者：方泽民[1] 何文涛[1] 王升[1] 蔡兰军[1] 雒真龙[1] 张维娜[1] 周鸿敏[1] 陈忠华 明长生[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所教育部/卫生部器官移植重点实验室,武汉430030

出　　处：《中华器官移植杂志》2010年第3期141-143,共3页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金（30772052）

摘　　要：目的 检测激活的TH1类淋巴细胞标记分子“T淋巴细胞免疫球蛋白域及粘蛋白域蛋白-3（Tim-3）”在受者体内不同部位T淋巴细胞上表达的变化，探讨其与急性排斥反应的关系。方法建立小鼠同基因和异基因心脏移植模型（简称：同基因组和异基因组）；移植术后第3和第6天，分离和制备两组受者外周血、脾脏、引流淋巴结和移植心内淋巴细胞悬液，采用流式细胞仪检测Tim-3阳性细胞在CD4＋和CD8＋T淋巴细胞中的比值。结果两组受者术后外周血和脾脏内Tim-3＋／CD4＋以及Tim-3＋／CD8＋的比值比较，差异均无统计学意义（P〉0．05）。与同基因组比较，异基因组引流淋巴结内Tim-3＋／CD4＋比值轻度升高（P〈0．05）；但异基因组术后第6天与第3天比较，差异无统计学意义（P〉0．05）。与同基因组比较，移植心内Tim-3＋／CD4＋和Tim-3＋／CD8＋比值均显著升高（P〈0．01）；异基因组术后第6天与第3天比较，移植心内Tim-3＋／CD4＋和Tim-3＋／CD8＋比值也显著升高（P＜0．01）。结论小鼠异基因心脏移植受者引流淋巴结和移植心内T淋巴细胞上Tim-3的表达升高与急性排斥反应的进展动态相关。Objective To explore the expression level of Tim-3, the marker of activated TH1 cells, in T lymphocytes in different sites from recipients with acute rejection. Methods The model of cervical heterotopic heart transplantation was established in mice. Two groups were set up： the isograft group （C57BL/6→C57BL/6） and the allograft group （Balb/c→C57BL/6）. Lymphocytes were isolated from peripheral blood, spleens, draining lymph nodes and grafts 3 or 6 days after transplantation. The expression of TIM-3 in CD4＋and CD8＋ T subsets was detected by flow cytometry. Results There was no significant difference in Tim-3＋/CD4＋ and Tim-3＋/CD8＋ ratio in peripheral blood or spleens between two groups. As compared with the isograft group, the proportion of Tim-3＋/CD4＋ cells was slightly elevated in draining lymph node （P〈0. 05）, but the percentage of Tim3＋/CIM＋ cells had no significant change between 3 days and 6 days in the allograft group （P〈 0. 05）. The expression of Tim3 in CD4＋ and CD8＋ of graft infiltrating T cells was obviously increased in allograft group （P〈0. 01 ）, and it was significantly （P〈0. 01 ） utvregulated on the 6th day as compared with that on the 3rd day. Conclusion The dynamic changes of Tim-3 expression in T lymphocytes in draining lymph node and graft were correlated with the progression of acute rejection in mice.

关 键 词：TIM-3 心脏移植 移植物排斥 T淋巴细胞 

分 类 号：R654.2[医药卫生—外科学]