The effects of gold nanoparticles on the proliferation, differentiation, and mineralization function of MC3T3-E1 cells in vitro  

作　　者：LIU DanDan ZHANG JinChao YI ChangQing YANG MengSu 

机构地区：[1]Chemical Biology Laboratory, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China [2]Key Laboratory of Biochip Research, Shenzhen Research Institute of City University of Hong Kong, Shenzhen 518057, China [3]Department of Biology and Chemistry, City University ofHong Kong, Hong Kong, China

出　　处：《Chinese Science Bulletin》2010年第11期1013-1019,共7页

基　　金：supported by the Key Laboratory Funding Scheme of Shenzhen Municipal Government;the AOE about MERIT project from University Grant Committee of Hong Kong (Grant No. AOE/P-04/2004);the Natural Science Foundation of Hebei Province (Grant No. B2009000161)

摘　　要：This study has investigated the effects of gold nanoparticles (Au NPs) on the proliferation, differentiation, and mineralization of a murine preosteoblast cell line MC3T3-E1 in vitro. The results show that Au NPs with diameters of both 20 and 40 nm promoted the proliferation, differentiation, and mineralization of MC3T3-E1 cells in a time- and dose-dependent manner at the concentra-tions of 1.5×10-5, 3.0×10-5, and 1.5×10-4 μmol/L. The reverse transcriptase polymerase chain reaction (RT-PCR) indicates that the expressions of runt-related transcription factor 2 (Runx2), bone morphogenetic protein 2 (BMP-2), alkaline phosphatase (ALP), and osteocalcin (OCN) genes increased after the 20 and 40 nm Au NP treatments, and the expression levels were higher than those of the NaF group. The above results suggest that Au NPs have the potential to promote the osteogenic differentiation and miner- alization of MC3T3-E1 cells and the particle size plays a significant role in the process. Runx2, BMP-2, ALP, and OCN genes may interact with each other, further stimulating the osteogenic differentiation of MC3T3-E1 cells.This study has investigated the effects of gold nanoparticles （Au NPs） on the proliferation, differentiation, and mineralization of a murine preosteoblast cell line MC3T3-E1 in vitro. The results show that Au NPs with diameters of both 20 and 40 nm promoted the proliferation, differentiation, and mineralization of MC3T3-E1 cells in a time- and dose-dependent manner at the concentrations of 1.5×10^-5, 3.0×10^-5, and 1.5×10^-4 μmol/L. The reverse transcriptase polymerase chain reaction （RT-PCR） indicates that the expressions of runt-related transcription factor 2 （Runx2）, bone morphogenetic protein 2 （BMP-2）, alkaline phosphatase （ALP）, and osteocalcin （OCN） genes increased after the 20 and 40 nm Au NP treatments, and the expression levels were higher than those of the NaF group. The above results suggest that Au NPs have the potential to promote the osteogenic differentiation and miner- alization of MC3T3-E1 cells and the particle size plays a significant role in the process. Runx2, BMP-2, ALP, and OCN genes may interact with each other, further stimulating the osteogenic differentiation of MC3T3-E1 cells.

关 键 词：金纳米粒子 细胞增殖 成骨分化 矿化 体外 核心结合因子Α1 逆转录聚合酶链反应 骨形态发生蛋白 

分 类 号：Q26[生物学—细胞生物学]