一氧化碳释放分子对重症急性胰腺炎肺损伤保护作用的实验研究  

An experimental study on protective effect of carbon monoxide releasing molecule on severe acute pancreatitis induced lung injury

作　　者：陈平[1] 王文闻[2] 王刚[1] 陈华[1] 孙备[1] 姜洪池[1] 

机构地区：[1]哈尔滨医科大学附属第一医院肝胆胰外科,150001 [2]哈尔滨医科大学附属第一医院肿瘤科,150001

出　　处：《中华肝胆外科杂志》2010年第3期196-199,共4页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的探讨一氧化碳释放分子（CORM-2）对大鼠重症急性胰腺炎（SAP）肺损伤的保护作用及机制。方法30只雄性Wistar大鼠随机分为3组（n=10）：sham组、SAP组、CORM2组。以3．5％牛磺胆酸钠逆行注射胰胆管的方法制作SAP模型。CORM-2组于SAP造模0．5h后经阴茎背动脉注射CORM-2（8mg／kg）。各组均于造模6h后取材，测定血清肿瘤坏死因子-α（TNF-α）含量；采用RT—PCR法检测肺组织细胞因子诱导的中性粒细胞趋化因子（CINC）及细胞间黏附分子-1（ICAM-1）mRNA的表达；同时检测肺组织髓过氧化物酶（MPO）活性、湿/于重比及对肺脏进行病理学评分。结果与SAP组相比，CORM-2组血清TNF—α水平、肺组织病理损伤程度、湿／干重比、MPO活性、CINC及ICAM-1 mRNA的表达均显著降低（P〈0．05）。结论应用CORM-2能够降低血清TNF—α水平、下调肺组织CINC及ICAM-1 mRNA的表达，进而有效地抑制肺组织中性粒细胞的大量浸润，从而对SAP肺损伤起到明显的保护作用。Objective To investigate the protective effect of carbon monoxide releasing molecule （CORM-2） on severe acute pancreatitis （SAP） induced lung injury in rats. Methods Thirty male Wistar rats were randomly divided into three groups （n= 10） including sham group, SAP group and CORM-2 group. The model of SAP was induced by retrograde infusion of 3.5% sodium taurocholate into the panereatobiliary duct. CORM-2 （8 mg/kg） was infused through the dorsal artery of penis 0.5 h after establishing the model of SAP in CORM-2 group. All animals were sacrificed 6 h after SAP induction. The serum level of tumor necrosis facto-α （TNF-α）, pulmonary cytokine induced neutrophil chemoattractant （CINC）, intercellular adhesion molecule-1 （ICAM-1） mRNA expression as well as the activity of myeloperoxidase （MPO） in the lung were examined in each group. The wet/dry ratio of the lung was also determined. The lung was scored on the basis of pathological changes. Results Compared with SAP group, pulmonary over-expression of CINC and ICAM-1 mRNA was obviously inhibited in CORM-2 group and the serum TNF-α level, the MPO activity in lung tissue, the wet/dry ratio of lung, and the pathological score of lung injury were significantly lower （P〈0.05）. Conclusion Administration of CORM-2 can remarkably reduce the severity of SAP induced lung injury through inhibiting the over-expression of CINC and ICAM-1 mRNA, down-regulating the serum TNF-α level and subsequently decreasing pulmonary neutrophil infiltration.

关 键 词：胰腺炎 一氧化碳释放分子 肺损伤 细胞因子诱导的中性粒细胞趋化因子 细胞间黏附分子-1 

分 类 号：R657.51[医药卫生—外科学]