一种酚氧化酶原组织定位的胶体金标记免疫电镜方法  

A method for tissue localization of prophenoloxidase based on colloidal gold labeled immunoelectron microscopy

作　　者：冯从经[1] 吕文静[1] 

机构地区：[1]扬州大学园艺与植物保护学院植物保护系

出　　处：《昆虫知识》2010年第2期414-418,共5页Entomological Knowledge

基　　金：国家自然科学基金(30571248);"973"计划(2006CB102002);扬州大学科技创新培育基金(2008CXJ028)

摘　　要：以亚洲玉米螟Ostrinia furnacalis Guenée5龄幼虫的中肠和体壁组织为材料,采用Epon812常规包埋方法,以作者实验室制备的酚氧化酶原多克隆抗体为一抗、胶体金标记的羊抗鼠IgG为二抗,采用醋酸双氧铀-柠檬酸铅双染色体系,建立一种胶体金标记的特异性强且超微结构保存较好的亚洲玉米螟幼虫体内中酚氧化酶原免疫电镜定位方法。A modified immunogold-labeling technique based on Epon 812 embedding specimen was used to localize prophenoloxidase（PPO） in the midgut and integument tissue from the 5th instar larvae of Ostrinia furnacalis Guenée.The mouse anti-O.furnacalis PPO serum prepared in our laboratory was used as primary antibody and colloidal gold labeled goat anti-mouse IgG as the secondary antibody.Bis（acetato） dioxouranium and lead citrate were used in the staining system.Both the labeling specific and resolution of electron microscopy were improved.

关 键 词：亚洲玉米螟 酚氧化酶原 胶体金 免疫电镜 

分 类 号：Q503[生物学—生物化学]