波长转换RP-HPLC法同时测定茯苓不同部位中5种三萜酸含量  

RP-HPLC simultaneous determination of five triterpenoid acids in different parts of Poria cocos by UV wavelengths switch

作　　者：车爽[1] 李清[2] 霍艳双[2] 陈晓辉[2] 毕开顺[2] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《药学学报》2010年第4期494-497,共4页Acta Pharmaceutica Sinica

摘　　要：建立同时测定茯苓不同部位中去氢土莫酸、猪苓酸C、3-表去氢土莫酸、去氢茯苓酸和茯苓酸含量的方法。采用紫外-波长转换检测的RP-HPLC法:色谱柱为KromasilC18柱(250mm×4.6mm,5μm);流动相为乙腈(A)-0.05%磷酸水溶液(B)系统,梯度洗脱(0～5min,60%A～64%A;5～35min,64%A～65%A;35～35.01min,65%A～73%A;35.01～53min,73%A);流速为1.0mL.min-1;检测波长为0～48min,241nm(去氢土莫酸、猪苓酸C、3-表去氢土莫酸、去氢茯苓酸),48～55min,210nm(茯苓酸)。去氢土莫酸、猪苓酸C、3-表去氢土莫酸、去氢茯苓酸和茯苓酸分别在30.5～610.0μg·mL-1(r=0.9996)、12.66～253.2μg·mL-1(r=0.9995)、2.99～59.7μg·mL-1(r=0.9997)、6.13～122.5μg·mL-1(r=0.9995)、11.3～226.0μg·mL-1(r=0.9995)内浓度与峰面积呈良好的线性关系;方法回收率分别为98.5%(RSD=1.9%)、99.4%(RSD=1.7%)、97.9%(RSD=1.2%)、96.7%(RSD=2.5%)和97.9%(RSD=2.3%)。结果表明,本方法简便、准确、重现性好,为茯苓药材的质量控制提供了依据。To establish a method for simultaneous determination of dehydrotumulosic acid,polyporenic acid C,3-epi-dehydrotumulosic acid,dehydropachymic acid and pachymic acid in Poria,a RP-HPLC method detected by UV wavelengths switch had been developed,including 210 nm （48-55 min） for pachymic acid and 241 nm （0-48 min） for dehydrotumulosic acid,polyporenic acid C,3-epi-dehydrotumulosic acid,dehydropachymic acid,separately. The system consisting of a Kromasil C18 column （250 mm × 4.6 mm,5 μm） and a mixture of acetonitrile and 0.05% phosphate acid as the mobile phase was adopted; The flow rate was 1.0 mL.min-1. The linear response range was 30.5-610.0 μg·mL^-1 （r = 0.999 6） for dehydrotumulosic acid,12.66-253.2 μg·mL^-1 （r = 0.999 5） for polyporenic acid C,2.99-59.7 μg·mL^-1 （r = 0.999 7） for 3-epi-dehydrotumulosic acid,6.13-122.5 μg·mL^-1 （r = 0.999 5） for dehydropachymic acid and 11.3-226.0 μg·mL^-1 （r = 0.999 5） for pachymic acid. The average recoveries of these compounds were 98.5% （RSD = 1.9%）,99.4% （RSD = 1.7%）,97.9% （RSD = 1.2%）,96.7% （RSD = 2.5%） and 97.9% （RSD = 2.3%）,respectively. The method is simple,accurate and reproducible for quality control of Poria.

关 键 词：茯苓 三萜酸 药用部位 产地 

分 类 号：R917[医药卫生—药物分析学]