应用动物活体生物发光技术观察紫杉醇混合胶束的抑瘤效果  

Study on the anti-tumor effect of paclitaxel mixed micelle by using in vivo optical imaging technique

作　　者：邰文[1] 孙敏敏[1] 刘楠[1] 黄志起[1] 聂尚海 郝艳丽[1] 张小宁[1] 

机构地区：[1]清华大学医学院,北京100084 [2]北京东胜创新生物科技有限公司,北京100085

出　　处：《药学学报》2010年第4期530-534,共5页Acta Pharmaceutica Sinica

基　　金：国家重大科学基础研究计划项目(2009CB903303)

摘　　要：活体动物成像技术是近年来发展成熟并得到认可的一种新型影像检测技术,其突出优越性是可以对活体病灶的形态大小进行在体无损伤直观准确检测。活体动物成像技术的基本原理是透过体表,接收动物体内物质所发出的荧光或可见光显像，因此检测中需要对病灶部位（或药物）进行造影标记。In vivo tumor imaging technique method based on bioluminescence principle was established to evaluate the anti-tumor effect of paclitaxel mixed micelle （PMM）. MDA-MB-231 tumor cells with luciferase reporter vectors were firstly implanted into nude mice,and subsequently the luciferase substrate was regularly injected during intraperitoneal administration of PMM. Then the tumor size,growth and the intensity of light signals were monitored with in vivo imaging technique. The method of luciferase tumor in vivo imaging could be real-time,reliable and exact in labeling and reflecting the growth of tumors,and the observed results were consistent with that by conventional method,so it would be a feasible approach to study anti-tumor effect of drugs. The anti-tumor effect of paclitaxel mixed micelle was observed by this method,and the results showed that this formulation could inhibit growth of tumor,and the anti-tumor rate of it was about 85%.

关 键 词：活体动物内光学成像 生物发光 荧光素酶 混合胶束 紫杉醇 

分 类 号：R943[医药卫生—药剂学]