17-β雌二醇提高巯醇抗氧化物(酶)治疗脊髓损伤的作用  

17-β estradiol reduces spinal cord injury of rats through raising thiol antioxidants

作　　者：边立功[1] 刘承杏[1] 李兴国[1] 马爱斌[2] 邹智荣[1] 李守民[1] 孙俊[1] 陆地[1] 

机构地区：[1]昆明医学院人体解剖学教研室,昆明650031 [2]昆明医学院第一附属医院神经内科,昆明650032

出　　处：《解剖学报》2010年第2期185-190,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金(30560170);云南省教育厅科学研究基金重点项目(07Z10392)资助

摘　　要：目的探讨17-β雌二醇治疗脊髓损伤的分子机制,为临床应用雌激素治疗脊髓损伤提供理论依据。方法成年雄性SD大鼠180只,采用改良的Allen法构建大鼠急性脊髓挫伤模型后经17-β雌二醇治疗,采用化学比色法测定脊髓组织中丙二醛(MDA)和内源性巯醇抗氧化物(酶):还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)含量;免疫组织化学分析凋亡相关因子Caspase-3、Bcl-2和Bax蛋白的表达变化。结果17-β雌二醇治疗组与脊髓损伤组比较,BBB评分明显增加;在多个时间点MDA含量明显降低;巯醇抗氧化物(酶)GSH、SOD和GSH-Px含量显著增加;Caspase-3和Bax表达的阳性细胞数明显减少;Bcl-2表达的阳性细胞数明显增加(P<0.05)。结论大剂量的17-β雌二醇治疗可能通过调控内源性巯醇抗氧化物(酶),提高肢体运动功能,抑制细胞凋亡,从而减轻脊髓损伤程度。Objective To examine the protective effects of 17-β estradiol on the experimental model of spinal cord injury （SCI） rats. Methods One hundred and eighty male Sprague Dawley （SD） rats, after Allen’s model, SD rats were divided into three groups： the sham group, the acute spinal cord injury （control groups） and the acute spinal cord injury supplying with 17-β estradiol treatment group. SCI was made by Allen’s weight dropping, impacting on the posteriors of spinal cord T10.The content of malonyldialdehyed （MDA）, glutathione （GSH）, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） were determined by chromatometry. The expressions of Caspase-3 and Bcl-2 family in the injured spinal cord were detected by immunohistochemical staining. Results The BBB scores at each time point in 17-β estradiol treatment group were significantly higher than that in SCI group （P〈0.05）. The contents of GSH, SOD, GSH-Px and the expression of Bcl-2 protein at the majority of time point in 17-β estradiol treatment group were significantly higher than that in SCI group（P〈0.05）, however, the MDA, Caspase-3 and Bax were markedly decreased （P〈0.05）. Conclusions This study suggests that 17-β estradiol administration might prevent the cells from SCI-induced apoptosis by triggering to reduce the oxidative stress.

关 键 词：17-Β雌二醇 脊髓损伤 巯醇抗氧化物酶 凋亡相关蛋白 化学比色法 免疫组织化学 大鼠 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]