低剂量棉酚和甾体激素联合用药对精母细胞凋亡和支持细胞吞噬作用的影响  

Effects of combined administration of low dose gossypol with steroid hormones on spermatocyte apoptosis and phagocytosis of sertoli cells

作　　者：常青[1] 钱晓菁[1] 许增禄[1] 张成侠[1] 

机构地区：[1]中国医学科学院基础医学研究所,北京协和医学院基础学院,人体解剖与组织胚胎学系

出　　处：《解剖学报》2010年第2期276-280,共5页Acta Anatomica Sinica

摘　　要：目的探讨低剂量棉酚和甾体激素联合用药对睾丸精母细胞数量、凋亡和支持细胞吞噬作用的影响。方法本实验采用大鼠喂服棉酚与甾体激素联合用药方式[棉酚12.5mg/(kg·d)+去氧孕烯125μg/(kg·d)+炔雌醇25μg/(kg·d)+十一酸睾丸酮100mg/(kg·d)],与单独喂服相同剂量的棉酚或激素的大鼠及喂服载体溶剂的大鼠相对照,用药4、6和8周取睾丸组织,应用体视学、原位缺口末端标记(TUNEL)和油红O染色的方法,观察精母细胞和球形精子细胞数量、凋亡和支持细胞吞噬作用。结果联合用药中,甾体激素成分可明显减少精母细胞和球形精子细胞的数量,精母细胞凋亡增加并同时伴有生精上皮油红O染色的增强。结论精母细胞凋亡并被支持细胞吞噬是联合用药抗生育机制之一。Objective To investigate effects of combined regimen of low dose gossypol with steroid hormones on apoptosis of spermatocyte and phagocytosis of sertoli cells. Methods Adult male rats were divided into four groups randomly, group GH： rats were fed orally with gossypol（GA） [12.5mg / （kg·d） ] and desogestrel（DSG）[125μg/（kg·d） ]/ethinyloestradil（E）[25μg/ （kg·d） ]/testosterone undecanoate（TU）[100mg/（kg·d） ]; group G： a single does of GA[12.5mg/（kg·d ） ] was given; group H： DSG[125μg/（kg·d） ]/E[25μg/（kg·d） ]/TU[100mg/（kg·d） ] were administered; group C： rats only received vehicle（1% methyl cellulose）. Testes from all the rats were removed at 4, 6 and 8 weeks after treatment for counting of spermatocyte and round spermatid using stereological assay, and for detecting apoptosis of spermatocyte and phagocytosis of sertoli cell by TUNEL and oil red O staining respectively. Results In GH group, the number of spermatocyte and round spermatid were reduced, while apoptosis of spermatocyte and staining of oil red O in seminiferous epithelium increased significantly. All changes were caused by steroid hormones in the combined regimen. Conclusion Induction of apoptosis of spermatocyte and then being phagocytosed by Sertoli cell is one of antifertility mechanisms of the combined regimen. 

关 键 词：棉酚 甾体激素 精母细胞 吞噬 体视学 大鼠 

分 类 号：R979.2[医药卫生—药品]