瘢痕疙瘩成纤维细胞培养中缺氧与缺氧诱导因子-1α表达的相关性  

Correlation of HIF-1α level with hypoxia in keloid fibroblasts

作　　者：郑旭东[1] 高建华[1] 胡振富[1] 鲁峰[1] 钱奕炜[1] 

机构地区：[1]南方医科大学南方医院整形美容外科,广州510515

出　　处：《中华医学美学美容杂志》2010年第2期113-115,共3页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的研究缺氧程度与瘢痕疙瘩中缺氧诱导因子-1α（hypoxia inducible factor，HIF－1α）基因表达的量化关系和关联性，进一步探讨缺氧通过HIF-1a途径促进异常瘢痕形成的机制。方法设立不同O2浓度组，在常氧及不同程度缺氧条件下培养瘢痕疙瘩成纤维细胞，应用Western印迹技术检测比较各组间HIF－1α基因蛋白的表达，进行数据处理和统计学分析。结果瘢痕疙瘩成纤维细胞在常氧（20％）、不同缺氧浓度（10％、5％、1％）下．HIF－1α蛋白表达相对含量（HIF－1α／β肌动蛋白）分别为0．007±0．006、0．133±0．006、0．537±0．015和0．903土0．021，表明缺氧系统中瘢痕疙瘩成纤维细胞HIF—1α蛋白表达明显上调。结论缺氧条件促进了瘢痕疙瘩中HIF-1α蛋白的表达，而且表达的量与缺氧程度呈正相关。缺氧通过HIF－1α基因途径与异常瘢痕的形成密切相关。Objective To investigate the correlation of HIF-1α and hypoxia in keloids fibroblasts, and to investigate the mechanism that hypoxia promotes abnormal scarring by HIF-1α pathway. Methods Keloid fibroblasts cultured in vitro were placed in an incubator with different O2 concentrations. After 24 h, the keloid fibroblasts were collected for further study. Western blotting was performed to detect the expression of HIF-1α in the keloid fibroblasts. Results Relative amounts of HIF-1α in keloid fibroblasts cultured under O2 concentrations at 20%, 10%, 5 % and 1% were 0.007±0. 006, 0. 133±0. 006, 0. 537±0. 015 and 0. 903±0. 021, respectively. It indicated that hypoxia could increase the expression of HIF-1α in keloid fibroblasts. Conclusions Hypoxia can induce the expression of HIF-1α in fibroblasts of keloids. Moreover, there still is a positive relation between hypoxia and the expression of HIF-1α. There-fore, a close relationship exists between abnormal scarring and HIF-1α pathway by hypoxia.

关 键 词：瘢痕疙瘩 成纤维细胞 缺氧诱导因子-1Α 缺氧 

分 类 号：R622[医药卫生—整形外科]