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作 者:魏旭冉[1] 杨军[1] 刘庆军[1] 王波[1] 周虹[1]
机构地区:[1]军事医学科学院野战输血研究所,北京市100850
出 处:《医学分子生物学杂志》2010年第2期93-98,共6页Journal of Medical Molecular Biology
基 金:国家高技术研究发展计划(863计划)(No.2006AA02A253)
摘 要:目的确定人血红素加氧酶-1(human heme oxygenase-1,hHO-1)在大肠埃希菌中的表达条件与纯化方法,利用纯化的蛋白制备具有中和活性的hHO—1多克隆抗体。方法将hHO-1原核表达质粒pMW172/hHO-1转人大肠埃希菌菌株BL21,通过改变摇床转速、诱导剂IPTG浓度和培养时间确定hHO-1蛋白的最佳可溶性表达条件;利用超声破碎、高速离心、分级盐析、分子筛层析等方法纯化hHO-1蛋白,建立体外HO-1活性测定方法检测hHO-1蛋白的活性;利用纯化的hHO-1活性蛋白作为抗原免疫新西兰兔,制备多克隆抗体;利用ELISA方法和Western印迹技术分别测定抗体的效价和特异性,通过HO-1活性测定检测抗体的中和活性。结果确定hHO-1最佳可溶性表达条件为:37℃、200r/min培养3h后,0.1 mmoL/LIPTG诱导培养4h。超声破碎菌体,上清经30%~40%盐析纯化及分子筛层析纯化,获得活性hHO-1蛋白,收得率为30.3%,纯化倍数为2.83倍,纯度为90%。制备的抗hHO-1的兔血清效价达到10^6,并能中和掉46%hHO-1的催化活性。结论为hHO-1蛋白的表达和纯化以及多克隆抗体制备确立了可行的技术方案;获得了高纯度活性hHO-1蛋白及hHO-1多克隆抗体,为HO-1功能、结构研究,以及相关疾病研究奠定了基础。Objective To define optimal expression condition in E. coli and purification methods of human HO-1 (hHO-1), and to prepare neutralizing antibody using the purified protein. Methods pMW172/hHO-1 plasmid coding hHO-1 was transformed into E. coli BL21. Ex- pression condition was optimized with different concentration of IPTG, rotation speed and time of shaking, hHO-1 protein purification of was performed by ultrasonication, high-speed centrifugation, salt fractionation and sieve chromatography, hHO-1 activity was examined by an established HO-1 in vitro activity assay. Antibodies against hHO-1 were prepared by immunizing New-Zealand rabbits with purified hHO-1. The titer and specificity of hHO-1 antibodies were determined by ELISA and Western Blot respectively, and the neutralizing activity of hHO-1 antibodies was determined by HO-1 activity assay. Results The optimal expression condition of hHO-1 was determined as 3 h cul- ture of E. coli BL21 at 200 r/min, 37℃ and subsequent d h induction by 0. 1 mmol/L IPTG. The purified HO-1 was obtained by 35 %-55 % salt fractionation and chromatography, with final purity of 90%, purification fold of 2. 83 and yield rate of 30. 3%. The polyclonal antibody specific to h HO-1 showed neutralizing activity, with high titer up to 10^6. Conclusions We established feasible experimental procedures for expression and purification of hHO-1 as well as the preparation of the polyclonal antibody specific to hHO-1. Our purified hHO-1 protein and specific hHO-1 polyclonal antibody are provided for study of HO-1 function and structure and HO-1 related diseases.
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