三氯乙烯对L-02肝细胞中SET表达的影响  

SET expression alteration in L-02 liver cells treated with trichloroethylene

作　　者：蒋英芝[1,2] 刘建军[2] 周桂凤[1] 张兵[2] 郑波[2] 黄爱君[2] 

机构地区：[1]湖南师范大学医学院预防医学系,湖南长沙410006 [2]深圳市疾病预防控制中心,广东深圳518020

出　　处：《癌变．畸变．突变》2010年第2期81-85,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(30972454;30571557);深圳市科技计划重点项目(200801010)

摘　　要：目的:研究不同剂量三氯乙烯(TCE)对L-02肝细胞中SETmRNA和蛋白水平表达的影响,为探索TCE致肝损伤的机制提供实验依据。方法:分别用0.5%二甲基亚砜(DMSO)、3mmol/LTCE及10mmol/LTCE处理L-02肝细胞24h后,采用实时荧光定量PCR(real-time quantitative PCR)技术和蛋白印迹(Western blotting)技术分别检测L-02肝细胞中SETmRNA和SET蛋白的表达。结果:与DMSO对照组相比,TCE处理组SETmRNA、SET蛋白表达均显著上调(P均<0.01),其10mmol/LTCE处理组的SET蛋白表达高于3mmol/LTCE处理组(P<0.05),呈剂量依赖关系。结论:TCE可诱导L-02肝细胞中SET表达上调,提示SET可能在TCE对肝细胞毒性效应中起着重要作用。OBJECTIVE：To investigate the effects of different doses of trichloroethylene（TCE） on the expression alteration of SET gene at both the mRNA and protein levels, in order to provide some clues for the liver-injury mechanism of TCE. METHODS： Real-time quantitative PCR and Western blotting were employed to detect the mRNA and protein levels of SET in L-02 liver cells, which were separately treated with DMSO, 3 mmol/L and 10 mmol/L TCE. RESULTS： Compared with the control group, the mRNA and protein levels of SET were both significantly up-regulated （P0.01） in the L-02 liver cells treated with TCE, and the expression of SET protein in the 10 mmol/L TCE group was higher than 3 mmol/L TCE group, showing a dose-dependent relationship. CONCLUSION： TCE could induce the expression of SET at both the mRNA and protein levels in a dose-dependent manner, which indicated that SET might play an important role in the hepatotoxicity of TCE.

关 键 词：三氯乙烯 SET 蛋白印迹 实时荧光定量PCR 

分 类 号：R136.3[医药卫生—劳动卫生]