六棱菊提取物对醋氨酚致小鼠肝损伤的影响  

Laggera alata extract prevents acetamidophenol-induced liver damage in mice

作　　者：伍义行[1] 郝冰洁[1] 胡少青[1] 施树云[2] 王建国[3] 杨雷香[2] 赵昱[2] 

机构地区：[1]中国计量学院生命科学学院药学系,浙江省杭州市310018 [2]浙江大学药学院,浙江省杭州市310053 [3]浙江大学医学院附属第一医院传染病诊治国家重点实验室,浙江省杭州市310003

出　　处：《世界华人消化杂志》2010年第7期711-715,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30701049;浙江省实验动物科技计划基金资助项目;No.2008F80016~~

摘　　要：目的:探讨六棱菊提取物(LAE)对药物性肝损伤的抑制作用.方法:采用FolinCiocalteu和HPLC方法对LAE的主要成分进行定量分析;通过预防给药探讨LAE对大剂量(1000mg/kg)醋氨酚引起的小鼠中毒死亡的影响;运用醋氨酚引起的小鼠急性肝损伤模型评价LAE大(200mg/kg)、中(100mg/kg)、小(50mg/kg)剂量对药物性肝损伤的抑制作用.结果:FolinCiocalteu法测定结果表明LAE的总酚含量为56.5gGAE/100g提取物;HPLC分析结果显示LAE的主要成分为是二咖啡酰奎尼酸类化合物,其含量为53.0%.醋氨酚中毒保护试验表明,LAE在100mg/kg剂量下对大剂量醋氨酚所致小鼠死亡有明显抑制作用.药效评价结果显示:LAE在50、100和200mg/kg剂量下灌胃给药,均能明显降低肝损伤小鼠血清AST和ALT水平,其中100和200mg/kg剂量具有显著性影响;病理组织学检测表明,模型组小鼠肝组织呈现以中央静脉为中心的放射状坏死和脂肪变性,LAE大、中、小剂量组小鼠肝损伤均有一定程度的改善.结论:六棱菊对醋氨酚所致的药物性肝损伤具有较强的抑制作用.二咖啡酰奎尼酸类化合物可能是六棱菊保肝作用的主要物质基础.AIM： To investigate the protective effects of Laggera alata against acetamidophenol-induced liver damage. METHODS： The main components of Laggera alata extract （LAE） were quantitatively analyzed by the Folin-Ciocalteu method and high-pressure liquid chromatography （HPLC）. Before mice were intragastrically given high-dosage acetamidophenol （1000 mg/kg）, different doses of LAE （50, 100 and 200 mg/kg） was intragastrically administered to investigate its preventive effect against acetamidophenol-induced liver damage. RESULTS： Quantitative analysis of LAE by the Folin Ciocalteu method showed that LAE contains a high content of phenolic compounds （52.6 g GAE/100 g extract）. The HPLC analysis indicated that dicaffeoylquinic acids are the major components of LAE, whose content amounts to 53%. LAE at a dose of 100 mg/kg could obviously protect mice from acetamidophenol-induced death. LAE at doses of 50, 100 and 200 mg/kg （especially 100 and 200 mg/kg） significantly reduced serum AST and ALT levels in mice treated with acetamidophenol. Furthermore, LAE at all doses could improve acetamidophenolinduced hepatic injury. CONCLUSION： LAE has potent protective effect against acetamidophenol-induced liver injury in mice. Dicaffeoylquinic acids may be the active ingredients in LAE that are responsible for the hepatoprotective effects of LAE.

关 键 词：六棱菊 二咖啡酰奎尼酸类化合物 醋氨酚 保肝作用 

分 类 号：R285.5[医药卫生—中药学]