中药提取液体外抗肠道念珠菌的作用  

Antifungal effect of extracts of 32 traditional Chinese herbs against intestinal Candida in vitro

作　　者：邓洁华[1] 王刚生[1] 马耀辉[1] 史敏[1] 李波[1] 

机构地区：[1]河北医科大学第二医院,河北省石家庄市050000

出　　处：《世界华人消化杂志》2010年第7期741-743,共3页World Chinese Journal of Digestology

摘　　要：目的:观察27种中药醇提液及5种中药提取油对肠道念珠菌(白念、热带、克柔氏)的体外抗菌活性.方法:采用试管药基法将27种中药醇提取液倍比稀释成浓度50-3.125g/L,中药提取油倍比稀释成5-0.0097mL/L,对肠道念珠菌进行MIC观察.结果:27种中药醇提取液中有6种(土槿皮、黄连、黄柏、五倍子、桂皮、丁香)MIC均值为1.56-3.25g/L,4种(广藿香、川芎、菖蒲、蛇床子)MIC均值12.5g/L,2种(五味子、小茴香)MIC均值50g/L,对念珠菌有不同程度的抑制作用,其他15种中药醇提取液无抑制作用.5种中药提取油(桂皮油、藿香油、丁香油、小茴香油、五味子油)对念珠菌均有较强的抑制作用.结论:6种中药醇提取液及5种中药提取油对念珠菌(白念、热带、克柔氏)均有较强的抑制作用.AIM： To investigate the antifungal activity of alcohol extracts of 27 traditional Chinese herbs （TCHs） and extracting oils from 5 TCHs against intestinal Candida （C. albicans, C. krusei, and C. tropicalis） in vitro. METHODS： The alcohol extracts of 27 TCHs were serially diluted to concentrations ranging from 50 to 3.125 g/L, while the extracting oils from 5 TCHs were serially diluted to concentrations ranging from 5 to 0.0097 mL/L. The minimum inhibitory concentrations （MICs） of these herb extracts against intestinal Candida were then determined. RESULTS： The alcohol extracts of 6 （cortex pseudolaricis, coptis chinensis, phellodendron amurense, Chinese gall, cassia, and clove）, 4 （patchouli, Chuanxiong, acorus calamus, and fructus cnidii）, and 2 （fructus schizandrae and fennel） TCHs hadmean MICs of 1.56 g/L to 3.25 g/L, 12.5 g/L, and 50 g/L, respectively. The alcohol extracts of the remaining 15 TCHs exhibited no antifungal effect. The extracting oils from 5 TCHs （cassia oil, Agastache rugosa oil, clove oil, small fennel oil, and fructus schizandrae oil） showed potent antifungal effect against intestinal Candida. CONCLUSION： The alcohol extracts of 6 TCHs and extracting oils from 5 TCHs have signifi cant antifungal activity against pathogenic Candida species （C. albicans, C. krusei, and C. tropicalis）.

关 键 词：肠道念珠菌 中药醇提取液 中药提取油 抗菌活性 

分 类 号：R285.5[医药卫生—中药学]