环氧化酶2抑制剂对大鼠术后腹腔粘连的影响  

Effects of celecoxib a COX-2 inhibitor on postoperative abdominal adhesion in rats

作　　者：魏光兵[1] 李徐奇[1] 王康[1] 段洪亮[1] 纪明明[1] 杨培彬[1] 

机构地区：[1]西安交通大学医学院第一附属医院普通外科,710061

出　　处：《中华普通外科杂志》2010年第3期235-238,共4页Chinese Journal of General Surgery

基　　金：陕西省科学技术研究发展计划基金资助项目（2007K12-02）

摘　　要：目的研究环氧化酶2（COX-2）的表达与术后粘连发生的关系，探讨选择性COX-2抑制剂塞来昔布在预防术后腹腔粘连中的作用。方法健康SD大鼠50只，随机分为5组（n=10），分别为阳性对照组、透明质酸钠组、塞来昔布小剂量组、塞来昔布大剂量组、阴性对照组。每组于造模术后第8天和第15天随机处死大鼠各5只，肉眼观察评估腹腔粘连程度，并取创伤部位肠管及腹膜Western-blot法检测组织COX-2蛋白含量。结果透明质酸钠组、塞来昔布小剂量组、塞来昔布大剂量组腹腔粘连程度以及创伤部位组织COX-2表达明显减低，与阳性对照组相比差异有统计学意义（P〈0．05）。透明质酸钠组与各塞来昔布组相比差异有统计学意义（P〈0．05）。结论COX-2选择性抑制剂塞来昔布通过降低创伤部位组织COX-2表达能减轻术后腹腔粘连。Objective To study the corelation between the expression of COX-2 and postoperative adhesions, and to determine the effect of COX-2 selective inhibitor, Celecoxib, on postoperative adhesion formation. Methods Fifty SD rats were randomly assigned to five groups each consisting of 10 rats. Study groups were as follows ： （A） positive control group, （B） sodiumhyaluronate group, （C） low dose Celecoxib group, （D） high dose Celecoxib group, （E） negative control group. Five rats in each group were treated accordingly for consecutive 8 days and 15 days respectively and sacrificed. After treatment, intra-abdominal adhesions were scored using a standard method. The adhesions tissure and injured peritonaeum were subjected to Western-blotting to detect the expression of COX-2. Results The level of postoperative adhesions and expression of COX-2 of sodiumhyaluronate group, low dose Celecoxib group, high dose Celecoxib group were lower than that of positive control group （ P 〈 0.05 ）. Sodiumhyaluronate treatment group was different from the two Celecoxib treated groups （ P 〈 0.05 ）. Conclusions Selective COX-2 inhibitor, Celecoxib, provides durable inhibition of intra-abdominal adhesions through an antiangiogenic COX-2 mechanism.

关 键 词：手术后并发症 环氧化酶2 疾病模型 动物 环氧化酶2抑制剂 组织粘连 

分 类 号：R965[医药卫生—药理学]