丹参酮对D-半乳糖-Aβ_(1-40)致痴呆大鼠海马内Aβ和AChE的影响  

Influence of Tanshinone on levels of β-amyloid and acetylcholinesterase in hippocampus of dementia rats induced by D-galactose combination with Aβ1-40

作　　者：李林[1] 茹立强[2] 

机构地区：[1]江汉大学医学院组织胚胎学教研室,湖北武汉430056 [2]华中科技大学同济医学院神经生物学系

出　　处：《中国老年学杂志》2010年第7期918-920,共3页Chinese Journal of Gerontology

基　　金：湖北省卫生厅科研基金项目(No.2005-JX2C35)

摘　　要：目的探讨丹参酮对D-半乳糖-Aβ1-40致复合痴呆模型大鼠海马内β-淀粉样蛋白(Aβ)和乙酰胆碱酯酶(AChE)表达的影响。方法实验动物随机分成AD模型组、丹参酮治疗组和溶媒对照组。采用免疫组织化学和酶组织化学方法,分别观察大鼠海马内Aβ和AChE表达的变化。结果AD模型组大鼠海马内Aβ-ir细胞数显著增多,平均光密度增强;AChE阳性神经纤维受损,光密度下降(含阳性面积百分比和光密度);与溶媒对照组比较差异显著(P<0.01)。丹参酮处理后,Aβ-ir细胞数明显减少,光密度也下降;AChE阳性神经纤维密度增强,与AD模型组比较差异有显著性意义(P<0.01)。结论丹参酮改善D-半乳糖-Aβ1-40致复合痴呆大鼠学习记忆障碍的作用机制,可能与降低海马内Aβ的表达而保护脑内胆碱能神经有关。Objective To investigate influence of tanshinone on levels of β-amyloid （Aβ） and acetylcholinesterase （AchE） in hippocampus of dementia model rats induced by D-galactose combination with Aβ1-40.Methods Rats were randomly divided into Alzheimer′s disease （AD） model, tanshinone treatment, and solvent control groups. The expression of Aβ and AChE in rats hippocampus were respectively observed by immunohistochemical and enzyme-histochemical methods. Results Aβ-ir cells count and the average optical density of AD rats in the hippocampus were obviously increased, AChE positive nervous fibers damaged, optical density of AChE positive nervous fibers were significantly decreased in AD model group compared with solvent control group. After treated with tanshinone, Aβ-ir cells count and the optical density obviously decreased, AChE-positive nervous fibers were significantly increased compared with AD model group. Conclusions Mechanism of tanshinone on improving learning and memory impairment dementia model rats induced by D-galactose combination with Aβ1-40 might be related to decreasing expression of Aβ in hippocampus and protecting cholinergic nerves in brain.

关 键 词：丹参酮 D-半乳糖 β-淀粉样肽蛋白 乙酰胆碱酯酶 海马 

分 类 号：R284.2[医药卫生—中药学]