高压氧对大鼠小肠缺血／再灌注损伤保护作用的研究  

Protective effects of hyperbaric oxygen on the injury of small intestine induced by ischemia reperfusion in rats

作　　者：贾春志[1] 周世辉[1] 王钢[1] 

机构地区：[1]中国医科大学附属第一医院急诊科,辽宁省沈阳110001

出　　处：《中华航海医学与高气压医学杂志》2010年第1期35-40,共6页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：沈阳市科学技术计划（92581）

摘　　要：目的在大鼠小肠缺血／再灌注（I／R）的不同时期应用高压氧（HBO）干预治疗，观察其对大鼠肠黏膜的保护作用。方法采用肠系膜上动脉夹闭／松夹闭方式建立小肠I／R损伤模型。50只大鼠随机分为5组（每组10只）：对照组（sham组）、I／R组、HBO预处理组（HBO—P组）、缺血期HBO组（HBO—I组）和再灌注期HBO组（HBO—R组）。采用酶联免疫吸附试验法（ELISA）检测肠道组织肿瘤坏死因子-α（TNF—α）；应用翻转囊袋法测定胰岛素（相对分子质量5000）黏膜-浆膜通过量以检测黏膜通透性；应用光镜和电镜观察肠道组织学改变并进行损伤评分。结果肠黏膜通透性依次为：Sham组最低，其次HBO—I组，再次HBO—P组（每2组比较P〈0．05），均明显低于HBO．R组和L／R组（P〈0．05）：HBO—R组略低于I／R组，但2组比较差异无统计学意义（P〉0．05）。肠道组织TNF-α含量依次是：Sham组最低，其次HBO—I组，再次HBO—P组（每2组比较P〈0．05），均明显低于HBO—R组和I／R组（P〈0．05）；HBO—R组略低于I／R组，但2组比较差异无统计学意义（P〉0．05）。组织学损伤评分依次是：Sham组最低，其次为HBO—I组，HBO—P组次之（每2组比较P〈0．05），均明显轻于HBO—R组和I／R组（P〈0．05）；HBO—R组略轻于I／R组，但2组比较差异无统计学意义（P〉0．05）。结论HBO预处理和缺血期应用HBO对大鼠肠黏膜I／R损伤有明显的保护作用，可以降低肠黏膜通透性和减少炎症反应；再灌注期应用HBO虽然对肠黏膜有一定的保护作用，但效果不明显。Objective To investigate protective effects of hyperbaric oxygen （HBO） on small intestine mucous membrane of SD rats during different stages of ischemia reperfusion （I/R）. Methods The animal model of small intestine ischemia reperfusion was developed by clamping the superior mesenteric artery （SMA） for 60 rain. Fifty male SD rats were randomly divided into 5 groups （n = 10） , i.e. the sham group, the I/R group, the HBO pretreated group before ischemia （HBO-P）, the HBO treatment group during ischemia （ HBO-I）, and the HBO treatment group during reperfnsion （HBO-R）. Levels of TNF-α in the intestinal tissue were measured by using enzyme -linked immunosorbent assay method （ELISA）. To assess intestinal mucosal barrier function, inverted gut sacs were prepared to measure the mucosal-to-serosal passage of insulin （molecular weight is 5000 ）. Intestinal histological changes were observed by using optical microscope and electron microscope. Injury scores were made for further assessment. Results Mucosal permeability of the intestine following ischemia reperfusion came in the following order： the sham group being the lowest, next the HBO-I group, and then the HBO-P group. Differences could be seen, when comparisons were made between the two groups （P〉0.05）. TNF-α levels in the intestine were in the following succession： the sham group being the lowest, next the HBO-I group, and then the HBO-P group （P 〈 0.05 ）. However, levels of TNF-α in the first two groups were obviously lower than those of the HBO-R and I/R groups （ P 〈 0.05 ）. And TNF-α level of the HBO-R group was slightly lower than that of I/R group, but without statistical difference （ P 〉 0.05）. Histological injury scores of the intestinal tissue in HBO-I were significantly lower than those in the HBO-P group （P 〈 0. 05）, when a comparison was made between the HBO-R and I/R groups （P 〈 0.05）, without significant statistical differences between the HBO-R and I/R groups （ P 〉 0.05 �

关 键 词：高压氧 缺血/再灌注损伤 肠黏膜通透性 

分 类 号：R686[医药卫生—骨科学]