基于光诱导介电泳的单粒子操纵技术  

Single Micro-particle Manipulation Based on Light-induced Dielectrophoresis

作　　者：宋春峰[1,2] 易红[1,2] 倪中华[1,2] 

机构地区：[1]东南大学机械工程学院,南京211189 [2]东南大学江苏省微纳生物医疗器械设计与制造重点实验室,南京211189

出　　处：《机械工程学报》2010年第7期148-153,共6页Journal of Mechanical Engineering

基　　金：国家高技术研究发展计划(863计划;2009AA04Z310);国家自然科学基金(50675033;30770533)资助项目

摘　　要：在综合分析当前生物粒子微操纵技术的基础上,设计一种基于光诱导介电泳技术的微操纵芯片,并完成芯片关键部件——光电导薄膜的加工。在准静态电场条件下,对光斑操纵模式进行建模仿真,光斑光强服从高斯分布,光斑尺寸直接影响电场平方梯度的分布,随着光斑尺寸缩小电场平方的x向梯度峰值增大,而随着高度升高电场平方的x向梯度峰值急剧减小。最后通过光诱导介电泳测试平台对单粒子操纵性能进行试验验证。试验结果表明,在去离子水中,当电压峰—峰值为20 V,频率1 MHz时,粒子受负介电泳作用而远离光斑,并且在光斑模式下直径50μm的粒子移动的最大速度可达45μm/s;而在环型虚拟电极模式下能有效控制单粒子运动方向。Following a review of current micro-manipulation technologies of biological particles, a micro-manipulation chip based on the light-induced dielectrophoresis technology is designed. And the key component of the chip （the photoconductive film） is manufactured. Then the pattern of spot manipulation is modeled and simulated in the quasi-static electric field condition. The spot light intensity obeys Gaussian distribution. The size of spot directly influences the distribution of the gradient of the square of electric field. The gradient peak in x-direction of the square of electric field increases along with the decrease of the spot size, and it rapidly reduces along with the increase of the height. Finally, the single-particle manipulation is experimentally confirmed by the light-induced dielectrophoresis testing platform. Results show that the latex beads suspended in the de-ionized （DI） water is operated by the negative dielectrophoresis, when the value （peak-to-peak） of the applied voltage is 20 V and the AC frequency is 1 MHz, and the maximum speed of 50um particle in the spot pattern is 45 μm/s. Under the same condition the movement direction of the single particles can be controlled in the annular virtual electrode model.

关 键 词：光诱导介电泳 微粒操纵 有限元法 

分 类 号：TP271[自动化与计算机技术—检测技术与自动化装置]