巴马小型猪与人真皮的组织形态学和生物力学基本特征比较研究  

Comparative study on dermal histomorphologic and biomechanical properties between Bama miniature pig and human

作　　者：郑必祥[1] 彭代智[1] 左海斌[1] 何斌[1] 王丽华[1] 何升东[1] 王勇[1] 周新[1] 刘敬[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆400038

出　　处：《第三军医大学学报》2010年第8期754-758,共5页Journal of Third Military Medical University

基　　金：国家高技术研究发展计划(863计划,2006AA02A121);国家重点基础研究发展计划(973计划,2005CB522605)~~

摘　　要：目的通过比较研究巴马小型猪与人皮肤在真皮组织结构、胶原类型和生物力学性能方面的差异,寻找猪脱细胞真皮基质临床应用受限相关的组织结构及生物力学原因。方法收集巴马小型猪与人的背部全层皮肤样本,采用大体观察、HE染色、Masson三色染色、天狼猩红染色、VVG染色、扫描电镜及透射电镜等方法,显微照相后进行形态学观察和图像分析软件测量,同时利用材料试验机检测真皮生物力学性能。结果与人皮肤比较,光镜下可见巴马小型猪皮肤的真皮胶原纤维束更粗[(39.29±10.17)μmvs(17.21±5.20)μm](P=0.000 3),而其间隙率更低[(19.01±1.55)%vs(32.36±1.28)%](P=0.029);真皮弹性纤维分布、Ⅰ型和Ⅲ型胶原含量及其比值也与人真皮有明显差异(P<0.05);扫描电镜显示巴马小型猪真皮的胶原纤维束排列紧密、束间孔隙小,透射电镜显示巴马小型猪真皮的胶原原纤维较细,横纹的周期更长;生物力学检测显示巴马小型猪真皮的最大应变[(0.52±0.05)mm/mm]低于人真皮[(0.80±0.06)mm/mm](P=0.003),而弹性模量[(69.65±12.16)MPa]和最大应力[(20.72±1.49)MPa]高于人真皮[分别为(44.23±4.63)、(11.85±0.19)MPa](P=0.028,P=0.001)。结论巴马小型猪的真皮与人真皮差异较大,从组织形态学的角度提示猪脱细胞真皮基质不是修复人体真皮缺损的最佳生物材料。Objective To compare the dermal tissue structure,content of difference types of collagen and biomechanical properties between Bama miniature pig and human skin,and to identify the histomorphologic reasons that limit the clinical application of porcine acellular dermal matrix.Methods The dorsal full-thickness skin samples were collected from Bama miniature pig and human,and then observed grossly.The sections of these samples were stained by hematoxylin and eosin（HE） staining,Masson＇s trichrome staining,sirius red staining and Verhoeff＇s Van Gieson（VVG） staining,and were examined under light microscopy.These samples were also observed by scanning electron microscopy（SEM） and transmission electron microscopy（TEM）.The relevant data of histological images were measured with image analysis software.In the meantime,these samples were detected by material testing machine for biomechanical properties.Results Compared with human dermis,Bama miniature pig had much thicker dermal collagen bundles [（39.29±10.17）μm vs（17.21±5.20）μm,P=0.000 3],and had significant decrease in the gap rate between these bundles [（19.01±1.55]% vs（32.36±1.28）%,P=0.029].The dermal collagen bundles of Bama miniature pig were densely arranged and had smaller pores by SEM,the collagenous fibrils were thicker,and had longer cyclical transverse striation by TEM.The distribution of elastic fiber,the contents of type Ⅰ and Ⅲ collagen and their ratio also had significant differences between human and porcine dermis（P〈0.05）.The maximum strain of Bama miniature pig dermis（0.52±0.05）mm/mm was smaller than that of human dermis [（0.80±0.06）mm/mm,P=0.003],while the elastic modulus（69.65±12.16）MPa and maximum stress（20.72±1.49）MPa of Bama miniature pig dermis were stronger than that of human dermis（44.23±4.63） and（11.85±0.19）MPa（P=0.028,P=0.001）.Conclusion The dermis of pig and human are quite different.It is suggested that the acelluar dermal matrix from pig is not an optimal biom

关 键 词：真皮 组织形态学 猪 人 基质 生物力学 

分 类 号：R322.99[医药卫生—人体解剖和组织胚胎学] R318[医药卫生—基础医学]