外侧下丘脑orexin能神经元区微注射腺苷对大鼠睡眠-觉醒周期的影响  

Microinjection of adenosine into orexinergic zone of lateral hypothalamus on sleep-wake cycle in rats

作　　者：寸艳萍[1] 夏建霞[1] 李洋[1] 胡志安[1] 

机构地区：[1]第三军医大学基础医学部生理学教研室,重庆市神经科学研究所,重庆400038

出　　处：《第三军医大学学报》2010年第8期767-769,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30670668);全军"十一五"科技攻关项目(08G080)~~

摘　　要：目的探讨外侧下丘脑orexin能神经元区微注射腺苷对SD大鼠睡眠-觉醒周期的影响。方法选用成年雄性SD大鼠14只,按3种不同的给药模式在外侧下丘脑orexin能神经元区分别微注射1、10、20 nmol/μl腺苷,以人工脑脊液为自身对照,然后采用脑电机同步记录大鼠的脑电和肌电活动,观察外侧下丘脑区微注射腺苷后大鼠睡眠-觉醒周期的变化。结果与人工脑脊液自身对照相比,在大鼠外侧下丘脑分别微注射1、10 nmol/μl和20 nmol/μl腺苷后的3 h内,大鼠觉醒时间明显减少到人工脑脊液自身对照的84%、62%和60%,并且大鼠睡眠时间均有不同程度的延长(P<0.05)。在微注射20 nmol/μl腺苷后,大鼠觉醒时间持续减少了3 h,并且与微注射1、10 nmol/μl腺苷后相比,其觉醒时间的减少和NREM/REM睡眠时间的增加也最明显。结论在外侧下丘脑orexin能神经元区微注射腺苷促进大鼠睡眠。Objective To investigate the effect of extragenous adenosine（AD） by microinjection in the orexinergic area of rat lateral hypothalamus（LH） on the sleep-wake cycle.Methods A total of 14 male SD rats were given 1,10 or 20 nmol AD and artificial cerebrospinal fluid（aCSF） as own control through three different models of microinjections into the LH.Their electroencephalogram（EEG） and electromyogram（EMG） were recorded by a polygraph recording instrument to observe the changes of sleep-wake after microinjections.Results AD administration to LH at doses of 1,10 and 20 nmol decreased the total amount of wakefulness by 84%,62% and 60% fold during that first 3-hour recording period and concomitantly increased non rapid eye movement（NREM） and REM sleep,when compared with aCSF treatment.The sleep-inducing effect was maintained even in 3 hour after the 20 nmol AD microinjection.The maximal sleep-inducing effect was obtained at the 20 nmol dose of AD administration into the orexinergic LH（P〈0.05）.Conclusion Unilateral microinjection of AD into orexinergic zone of LH promotes NREM/REM sleep of rats.

关 键 词：腺苷 外侧下丘脑 orexin能神经元 

分 类 号：R338.24[医药卫生—人体生理学] R338.63[医药卫生—基础医学]