糖尿病大鼠早期皮肤改变与血清炎性介质变化  

Early skin changes and serum inflammatory mediators in diabetic rats

作　　者：张晓菲[1] 郝飞[1] 杨景[2] 

机构地区：[1]第三军医大学西南医院:皮肤科,重庆400038 [2]第三军医大学西南医院:病理学研究所 [3],重庆400038

出　　处：《第三军医大学学报》2010年第8期787-789,共3页Journal of Third Military Medical University

基　　金：国家“十一五”攻关项目(2006BA702B01)~~

摘　　要：目的观察糖尿病(diabetes mellitus,DM)大鼠早期皮肤损害的组织病理特点与血清炎性介质的变化。方法将50只8～10周龄Wistar大鼠分为正常对照组(C组,n=25)、DM组(n=25);25只建立DM大鼠模型,其中3只死亡。两组于第4、8、12周末各处死4只,取背部皮肤标本,以观察DM大鼠皮肤早期改变;其余大鼠在第16周末抽取心脏血测定TNF-α、IL-6、C-RP的水平,并处死取皮肤,进行病理学观察及测量皮肤全层与真皮厚度。结果第16周末DM组的TNF-α(1.45±0.67)ng/ml、IL-6(135.05±43.39)pg/ml、C-RP(3.51±0.62)mg/L较正常对照组[分别为(0.86±0.60)ng/ml、(99.92±32.36)pg/ml、(2.54±1.31)mg/L]明显增高(P<0.05)。测微尺测量表明DM组大鼠4、8、12、16周皮肤全层均变薄,与正常对照组比较有明显差异(P<0.05,P<0.01)。DM组各时相点表皮厚度较正常对照组薄,但无统计学差异(P>0.05)。组织学观察:DM组表皮细胞层次欠清晰,真皮层胶原萎缩、肿胀、退化变性,皮下脂肪进行性萎缩。结论建立成功的DM大鼠动物模型皮肤表现为全层与皮下脂肪变薄、萎缩及血清炎症因子明显增高。Objective To observe the histopathological features of early skin lesions and serum inflammatory mediators changes in the diabetes mellitus（DM） rats.Methods A total of 50 Wistar rats at 8 to 10 weeks of age were divided into normal control group（C group,n=25） and diabetic group（DC group,n=25）.The rats of DC group were given a high-glucose and high-fat diet for 2 weeks to induce insulin resistance and then an intraperitoneal injection of 35 mg/kg streptozotocin.All 25 rats were identified to be diabetic models with their fasting blood glucose over ≥7.0 mmol/L.Four rats of C group or DC group were killed to take the skin tissues in the 4th,8th and 12th weekend.In the 16th week,the heart blood samples of the left 13 rats of C group and 10 rats of DC group were collected to measure TNF-α,IL-6 and C-RP levels,and the skin samples were also taken for pathological observation and full-thickness and dermal thickness measurement.Results In the 16th week,the TNF-α,IL-6 and C-RP levels of DC group were significantly higher than the normal group（P〈0.05）.Micrometer measurements indicated that the skin full-thickness of diabetic rats became thinner and thinner in the 4th,8th,12th and 16th weeks,with significant difference when compared with normal group（P〈0.01,P〈0.05）.The epidermal thickness of diabetic group were thinner compared with normal skin at all time points,but there was no statistical difference among them.Histological observations of the diabetic group showed that there were lesser clear epidermal cells,shrunk,swelling and degraded dermal layer of collagen,and progressive atrophy of subcutaneous fat.Conclusion The successful established diabetic rat models show that their skin full-thickness and subcutaneous fat became thinner and atrophy,with significantly higher serum inflammatory cytokines.

关 键 词：糖尿病 炎症因子 病理 皮肤厚度 

分 类 号：R587.2[医药卫生—内分泌] R751.02[医药卫生—内科学]