国产水溶性蜂胶对主要致龋链球菌及其耐氟菌株致龋性的影响  

Effect of propolis on cariogenesis of main cariogenic streptococci and their fluoride-resistant strains

作　　者：彭志庆[1] 林居红[1] 刘明方[2] 邓一平[2] 

机构地区：[1]重庆医科大学附属口腔医院预防科,重庆400015 [2]重庆医科大学实验教学中心,重庆400016

出　　处：《第三军医大学学报》2010年第8期798-801,共4页Journal of Third Military Medical University

基　　金：重庆市自然科学基金(CSTC2007BB5275)~~

摘　　要：目的了解国产水溶性蜂胶对变形链球菌(S.mutansATCC 25175,S.m)、远缘链球菌(S.sobrinus6715,S.s)及其耐氟菌株的生长抑制作用,以及对葡糖基转移酶(glucosyltransferase,GTF)的影响,探讨一种新的防龋途径。方法采用最小抑菌浓度(MIC)递增法对S.m、S.s进行氟化钠体外诱导耐氟菌株(S.m-FR、S.s-FR),采用液体稀释法观察水溶性蜂胶对S.m、S.m-FR、S.s、S.s-FR生长的影响;用酶化学分析方法检测蜂胶对GTF酶活性的影响。结果水溶性蜂胶对S.m、S.m-FR、S.s、S.s-FRMIC分别为0.39、0.78、0.20、0.39 g/L;最小杀菌浓度(MBC)分别为0.78、1.56、1.56、1.56 g/L;随着蜂胶浓度增高,葡糖基转移酶活性逐渐降低,6.25、3.13、1.56 g/L与0.78、0.39 g/L 2个浓度之间差异具有统计学意义(P<0.05),各浓度组与阳性对照组比较差异有统计学意义(P<0.05)。结论国产水溶性蜂胶能有效抑制变形链球菌、远缘链球菌及耐氟菌株的生长,对GTF具有较强的抑制作用。Objective To evaluate the effects of propolis on the growth of S.mutans ATCC 25175（S.m）,S.sobrinus 6715（S.s） and their fluoride-resistant strains（S.m-FR,S.s-FR）,and the change of their glucosyltransferase（GTF）.Methods S.m and S.s were induced with sodium fluoride by minimum inhibitory concentration（MIC） to induce fluoride-resistant strains（S.m-FR and S.s-FR）.Fluid dilution method was used to observe the effects of different levels of propolis on growth of S.m,S.m-FR,S.s and S.s-FR.Chemistry enzyme analysis was used to detect the influence of propolis on the enzyme activity of GTF.Results MIC of propolis for S.m,S.m-FR,S.s and S.s-FR were respectively 0.39,0.78,0.20 and 0.39 g/L.MBC of propolis for them were respectively 0.78,1.56,1.56,and 1.56 g/L.GTF of S.m,S.m-FR,S.s,and S.s-FR was decreased gradually with the increase of concentration of propolis.There were significant differences among total sample groups,and each experimental group and positive control group as well（P〈0.05）.Conclusion Propolis effectively inhibits the growth of S.m,S.m-FR,S.s and S.s-FR,and inhibits the GTF activity.

关 键 词：国产水溶性蜂胶 变形链球菌 远缘链球菌 耐氟菌株 葡糖基转移酶 

分 类 号：R282.74[医药卫生—中药学] R378[医药卫生—中医学]