湖北地区肋壳钉螺与光壳钉螺种群间亲缘关系的探讨  

RELATIVE RELATIONSHIP STUDY OF RIB-SHELL ONCOMELANIA AND SMOOTH-SHELL ONCOMELANIA IN HUBEI PROVINCE

作　　者：王少海[1] 何立[2] 康在彬 

机构地区：[1]湖北药检高等专科学校寄生虫学教研室,武汉430064 [2]湖北医科大学寄生虫学教研室

出　　处：《中国血吸虫病防治杂志》1998年第5期267-270,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金

摘　　要：应用聚丙烯酰胺凝胶垂直平板电泳对长江水系的湖北钟祥、蒲圻光亮钉螺和江陵、潜江、阳新助壳钉螺进行酯酶同工酶（EST）和苹果酸脱氢酶（MDH）电泳比较，结果显示：3地肋壳钉螺EST同工酶酶带数目、活性及Rf值完全一致；2地光亮钉螺与肋壳钉螺相比，酶谱特征也基本一致，EST同工酶仅1－3条弱带稍有差异；MDH同工酶仅蒲圻光亮钉螺与其它各地肋壳与光亮钉螺稍有差异，提示上述各地钉螺在种系进化上具有比较密切的亲缘关系。This paper reports a comparative study on the esterase (EST) isoenzyme and malate de hydrogenase (MDH) isoenzyme of Oncomelania snails by means of slab polyacrylamide gel electrophoresis. The smooth-shell Oncomelania were collected from Zhongxiang and Puqi while the rib-shell Oncomelania were from Jiangling、 Qianjiang and Yangxin of Hubei province. All these snails were dispersed in the Yangtze River system. The results showed that the rib-shell Oncomelania from these 3 conuties were entirely identical in number of EST enzyme bands,enzyme activity and Rf values. The very similar isoenzyme patterns of EST were shown between the smooth-shell Oncomelania and rib-shell Oncomelania. A very small difference was observed in MDH isoenzyme patterns between smooth-shell Oncomelania from Puqi and rib-shell Oncomelania from the other areas. These results indicate that the Oncome lania snails from the different conuties in Hubei province have very close relationship be tween each other.

关 键 词：钉螺 种群 同工酶 亲缘关系 肋壳钉螺 光壳钉螺 

分 类 号：Q959.212[生物学—动物学]