北方汉族慢性乙型肝炎患者TNF-α启动子区基因多态性的分析  

Analysis of TNF-α promoter region polymorphisms in chronic hepatitis B patients in north Han population

作　　者：孟艳英[1] 高冀蓉[1] 邹怀宾[1] 李卓[1] 刘旭华[1] 赵军[1] 陈煜[1] 段钟平[1] 

机构地区：[1]首都医科大学附属北京佑安医院人工肝中心,北京100069

出　　处：《胃肠病学和肝病学杂志》2010年第4期319-322,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家高技术研究发展计划(863计划)(2006AA02A410);首都医学发展科研基金(2005-1028)

摘　　要：目的探讨TNF-α启动子区基因多态性对宿主感染乙型肝炎病毒(hepatitis B virus,HBV)慢性化结局的关联。方法用病例-对照研究方法,291例慢性乙型肝炎患者作为病例组和212例乙型肝炎病毒自限性感染者作为对照组,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对TNF-α基因启动子区-238G/A、-857C/T、-863C/A位点进行基因分型。结果携带TNF-α-238 GA基因型、-857 CC基因型是HBV感染后宿主发展为慢性乙型肝炎的易感因素(P<0.05);3个位点组成的单体型-238G/-857C/-863A的频率在慢性乙肝组显著高于HBV自限感染组。结论TNF-α启动子区基因多态性可能对宿主感染HBV慢性化结局产生影响。Objective To determine whether the polymorphisms of tumor necrosis factor α（TNF-α） promoter region is associated with the outcomes of HBV infection. Methods The study adopted case-control design and candidate genes strategy.A total of 503 cases that came from northern Han population were enrolled in the study,including 291 chronic hepatitis B patients（CHB） and 212 spontaneously HBV cleared subjects（SC）.Polymorphisms of TNF-α promoter region were detected using polymerase chain reaction-restriction fragment length polymorphism（PCRRFLP）.The frequencies of the polymorphisms of TNF-α promoter gene in two groups were compared using pearson chi-square,Fisher exact test for P value,odds ratio（OR） and 95% confidence interval（CI） to determine the influence of host genetic factors on HBV infection outcomes. Results TNF-α-238GA genotype and TNF-α-857CC genotype were susceptible factors to chronic hepatitis B after HBV infection（P〈0.05）.Haplotype frequency of-238G/-857C/-863A in chronic hepatitis B patients was significantly higher than that in spontaneously HBV cleared subjects. Conclusion TNF-α promoter region polymorphisms are likely to play an important role in the outcome of HBV infection in northern Han population.

关 键 词：慢性乙型肝炎 TNF-Α基因 基因多态性 

分 类 号：R512.62[医药卫生—内科学]