大麻素CB2受体参与电针预处理诱导的延迟相脑保护作用  

Cannabinoid CB2 receptor participates in induced by electroacupuncture pretreatment the delayed tolerance to focal cerebral ischemia

作　　者：马磊[1] 侯丽宏[1] 赵昱[1] 王强[1] 朱萧玲[1] 朱正华[1] 赵宁侠[1] 贾济[1] 陈绍洋[1] 

机构地区：[1]第四军医大学西京医院麻醉科,西安710032

出　　处：《中华神经医学杂志》2010年第4期325-329,共5页Chinese Journal of Neuromedicine

基　　金：基金项目：国家自然科学基金（30572421,30772826）

摘　　要：目的探讨大麻素CB2受体在电针预处理诱导的脑缺血耐受中的作用。方法实验1：成年雄性SD大鼠40只按随机数字表法分为5组：大脑中动脉闭塞（MCAO）组、电针（EA）＋McA0组、CB2受体拮抗剂（AM630）＋EA＋MCAO组、AM630的溶剂（V）＋EA＋MCAO组和AM630＋MCAO组（n=8），线拴法制作MCAO模型，EA预处理在模型前2h给予，AM630或其溶剂在模型前5．5h给予，于模型后72h行神经功能评分和2，3．5．氯化三苯基四氮唑（TTC）染色检测脑梗死容积百分比的变化；实验2：成年雄性SD大鼠40只分组和处理同上，EA预处理在模型前24h给予，AM630或其溶剂在模型前27．5h给予，检测指标的时间和方法同实验1；实验3：成年雄性SD大鼠36只按照随机数字表法分为6组：对照组和EA后2、6、12、18、24h组（n=6）。后5组给予EA处理后在不同时间点应用RT—PCR和WesternBlot检测各组大鼠右侧大脑中CB2受体的表达。结果实验1：与MCAO组和AM630＋MCAO组比较，EA＋MCA0组、AM630＋EA＋MCAO组、v＋EA＋MCAO组大鼠神经功能评分增高，脑梗死容积百分比降低，差异有统计学意义fP〈0．05）：实验2：与MCAO组比较，EA＋MCAO组和V＋EA＋MCAO组大鼠神经功能评分增高，脑梗死容积百分比降低，与EA＋MCAO组比较，AM630＋EA＋MCAO组和AM630＋MCAO组大鼠神经功能评分降低，脑梗死容积百分比增高，差异均有统计学意义（P〈0．05）；实验3：与对照组比较。EA后18h组CB2受体mRNA水平增高，EA后24h组CB2受体蛋白增高，差异有统计学意义（P〈0．05）。结论CB2受体参与了电针预处理诱导的延迟相脑保护作用。Objective To investigate the effect of cannabinoid CB2 receptor on ischemic tolerance of rat models with focal cerebral ischemia induced by electroacupuncture （EA） pretreatment. Methods The first experiment was performed as follows： 40 adult male SD rats were randomized into 5 groups （middle cerebral artery occlusion [MCAO] group [vehicle], EA＋MCAO group, AM630 [the antagonist of CB2 receptor]＋EA＋MCAO group, V [the solvent of AM630]＋EA＋MCAO group and AM630＋MCAO group, n=8）. Suture method was employed to induce the MCAO models. Pretreatment with EA was given 2 h before the model making; pretreatment with AM630 or other solvents were given 5.5 h before the model making. The changes of infarction volume percentage were detected by 2,3, 5-triphenoltetrazolium chloride （TTC） staining and neurobehavioral scores were evaluated 72 h after the success of model making. In the second experiment, 40 adult male SD rats were performed the same treatment with the first experiment; pretreatment with EA was performed 24 h before the model making; pretreatment with AM630 or other solvents were performed 27.5 h before the model making; the measurements and detection times were the same with the first one. In the third experiment, 36 adult male SD rats were randomized into 6 groups （n=6）： control group and EA-treated groups （2, 6, 12, 18 and 24 h after the treatment）. RT-PCR and Western blotting were employed to detect the expression of CB2 receptor on the right side of rat brain. Results Compared with the vehicle and AM630＋MCAO groups, the EA＋MCAO group, AM630＋EA＋MCAO group and V＋EA＋MCAO group showed significantly higher scores of nerve function and a statistically lower percentage of infarction volume （P〈0.05）. Compared with the vehicle group, EA＋MCAO group and V＋EA＋MCAO group showed significantly higher neurobehavioral scores and a lower percentage of infarction volume （P〈0.05）; compared with the EA＋MCAO group, the AM630＋EA＋MCAO group and AM630＋MCAO group s

关 键 词：CB2受体 脑缺血 电针 预处理 

分 类 号：R245[医药卫生—针灸推拿学]