棉花亲环素基因(GhCYP1)克隆及在干旱胁迫下的表达分析  

Cloning of Cotton Cyclophilin Gene (GhCYP1) and its Expression under Drought-stress

作　　者：郭芳[1] 李艳军[1] 张新宇[1] 吴莉[1] 孙杰[1] 

机构地区：[1]石河子大学生命科学学院与农学院,新疆兵团绿洲生态农业重点实验室,石河子832003

出　　处：《分子植物育种》2010年第2期265-270,共6页Molecular Plant Breeding

基　　金：农业部转基因生物新品种培育重大专项(2008ZX08005)资助

摘　　要：本研究从已构建的棉花(Gossypium hirsutum)干旱胁迫抑制性差减杂交(suppressive subtractive hybridization,SSH)cDNA文库分离得到一个含有植物亲环素保守结构域的EST片段,测序并结合cDNA末端快速扩增(5'-RACE)技术获得了1个779bp的cDNA序列。序列分析表明,该cDNA5'非翻译区为70bp,3'非翻译区为187bp,并含有一个编码174个氨基酸蛋白的开放阅读框。Blast分析表明,该基因的编码产物为一个亲环素蛋白,将该基因命名为为GhCYP1,序列提交到GenBank,登录号为GQ292530。半定量RT-PCR分析表明,干旱胁迫处理后,该基因在叶片中的表达量迅速提高,并在胁迫2h达到最高,这一研究暗示该基因的表达与棉花抗旱胁迫相关。Based on a partial sequence of LEA （late embryogenesis abundant proteins） gene from cDNA library of Polygonum sibiricum Laxm. leaves, the LEA gene, designated as PsLEA （GenBank accession No. FJ478172.）, was cloned by using rapid amplification of cDNA end （RACE） technology, The acquired gene was 686 bp in full length, including 102 bp of 5＇ untranslated region and 131 bp of 3＇ untranslated region. The open reading frame （ORF） is 453 bp in length, encoding a deduced amino acid sequence of 150 residues. Quantitative fluorescent PCR （QF-PCR） results show that PsLEA gene was expressed in leaves and stems as well as underground stems of Polygonum sibiricum Laxm. under normal conditions, and the expression level was higher in stems than in leaves and underground stems. Under 3% NaHCO3 stress, the expression level is greatly affected and the expression patterns varied in different tissues, implying that PsLEA gene plays a role in the salt resistance of Polygonum sibiricum Laxm..

关 键 词：棉花 亲环素 干旱胁迫 

分 类 号：S562[农业科学—作物学]