转化生长因子-β1在糖尿病骨折延迟愈合大鼠中的表达  

The expression of transforming growth factor-β1 in the healing of rats＇ delayed union with diabetes

作　　者：赵锡武[1] 宫明智[1] 刘中浩[1] 武士清[1] 邢德国[1] 吴建军[1] 

机构地区：[1]山东大学第二医院骨外一科,济南250033

出　　处：《中华实验外科杂志》2010年第4期520-522,共3页Chinese Journal of Experimental Surgery

基　　金：山东省科技攻关计划资助项目（2007GG20002034）

摘　　要：目的观察转化生长因子-β1（TGF-β1）的表达，探讨其对糖尿病骨折延迟愈合的影响。方法70只大鼠随机分为对照组和链脲佐菌素按60mg／kg诱导的糖尿病组，血糖〉11．2mmol／L为诱导成功。均造成左侧胫骨骨折，于1、2、3、4、6、8周摄X片，取骨痂苏木素．伊红（HE）染色，免疫组织化学和酶联免疫吸附试验（ELISA）检测骨痂及血清TGF-β1。结果X片和HE染色均示实验组较对照组骨形成滞后；实验组4周TGF-β1表达达高峰，迟于对照组3周，3周灰度值：实验组189．59±2．76，对照组166．74±1．33（P〈0．05），3周血清含量：实验组（12．05±1．56）μg／L，对照组（17．48±0．56）vg／L（P〈0．05）。结论糖尿病大鼠骨折后血清及骨痂TGF-β1减少是致其延迟愈合的原因之一。Objective To observe the expression of transforming growth factor-β1 （ TGF-β1 ） during the healing of rats with diabetes and explore its influence on delayed union. Methods Seventy rats were divided randomly into experimental group and control group. Streptozotocin （60 mg/kg） was injected into the enteroeoelia of experimental group to induce diabetes model （ the blood sugar level 〉 11.2 mrnol/ L）. Nomal saline was given instead to the control group. All animals suffered left tibia fracture. Regular X-ray photographs were taken, bony callus was subjected to HE staining and the levels of TGF-β1 in the bony callus and serum were detected by immunohistoehemieal method and enzyme-linked immunosorbent assay （ELISA） respectively. Results As compared with the control group, the bone formation was siguificandy delayed in the experimental group at the 1st, 2nd, 3rd, 4th, 6th, 8th week both from X-ray and HE sections. Immunohistoehemieal staining and ELISA revealed that there was no significant difference in the levels of TGF-131 between two groups at the 3rd week. The gray scales at the 3rd week were 189.59 ±2. 76 in diabetes group and 166.74 ± 1, 33 in control group （ P 〈 0. 05 ） , respectively. The serum levels at the 3rd week were （12. 05 ±1.56）μg/L in diabetes group and （17.48 ±0. 56） μg/L in control group （P〈 0. 05 ） , respectively. Conclusion The decrease of TGF-β1 in both serum and bony callus is one of causes for poor fracture healing in rats with diabetes after fracture.

关 键 词：转化生长因子 糖尿病 骨折 延迟愈合 

分 类 号：R587.2[医药卫生—内分泌] R683[医药卫生—内科学]