污泥高温好氧消化过程的生物多样性分析  

Analysis of microbial diversity in thermophilic aerobic digestion of sewage sludge

作　　者：刘树根[1,2] 朱南文[1] 楼紫阳[1] 聂俊英[1] 

机构地区：[1]上海交通大学环境科学与工程学院,上海200240 [2]攀枝花学院生物与化学工程学院,攀枝花617000

出　　处：《环境科学学报》2010年第5期990-995,共6页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金项目(No.50878127)~~

摘　　要：模拟单级污泥高温好氧消化工艺进行批式试验,分析了不同消化时期污泥中挥发性固体物质(VSS)的去除率.同时,运用现代分子生物学手段对活性污泥中提取的细菌基因组DNA进行PCR扩增,扩增产物经变性梯度凝胶电泳(DGGE)分离,获得微生物群落的DNA特征指纹图谱,初步探讨了高温消化时微生物多样性对污泥稳定化的影响.结果表明:污泥高温好氧消化时,VSS降解速率较快,消化120h即可达到38%以上的去除率;温度对消化体系中微生物的种群结构影响较为明显,芽孢杆菌是高温(55℃)消化时的主要种群;消化过程中,体系内部优势微生物种群结构发生了较大改变,这种及时调整使整个消化体系能尽快适应外部环境变化,从而加快污泥稳定化进程.A batch experiment simulating single-stage thermophilic aerobic digestion for sewage sludge treatment was conducted to investigate the removal of volatile suspended solid （VSS） at different digestion stages. The microbial diversity in the digestion system and its effects on stabilization of sludge were studied using modern molecular biological techniques including bacterial genome extraction,PCR amplification and DGGE fingerprinting. Thermophilic aerobic digestion was effective in removing VSS in sludge,with up to 38% removal of VSS at a digestion time of 120 h. Temperature had a remarkable effect on microbial diversity,and Bacillus species were the dominant bacteria at 55℃. The change in the dominant microbial population allows the digestion system to accommodate to the external environment. The results obtained from this study indicate that thermophilic aerobic digestion could facilitate the stabilization of sludge.

关 键 词：污泥 高温好氧消化 生物多样性 16SrDNA PCR-DGGE 

分 类 号：X703[环境科学与工程—环境工程] X705