空间环境诱变甜椒基因组DNA变异的分子检测  

MOLECULAR DETECTION OF GENOMIC DNA VARIATION INDUCED BY SPACE ENVIRONMENT IN SWEET PEPPER (Capsicum annuum L.)

作　　者：谢立波[1] 郭亚华[1] 孟凡娟[2] 刘录祥[3] 王雪[1] 高永利[1] 

机构地区：[1]黑龙江省农业科学院园艺分院,黑龙江哈尔滨150069 [2]东北林业大学生命科学学院,黑龙江哈尔滨150040 [3]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程,北京100081

出　　处：《核农学报》2010年第2期254-258,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家863(2007AA100104);国家科技支撑计划(2008BAD97B06);航天育种工程项目(2006HT20012);农业部农业公益性行业科研专项(200803034);黑龙江省自然基金重点项目(ZD200818-01);黑龙江省自然基金项目(C200943)

摘　　要：为明确空间环境对甜椒后代的诱变影响,本研究以空间诱变的甜椒第4代及其地面对照为主要研究材料,采用RAPD技术对二者的基因组DNA进行了扩增分析,并对部分差异片段进行了测序。结果发现:空间诱变的甜椒后代与其地面对照变异情况相比,SP4的基因组DNA发生了变异,共检测出8个多态性位点,在对照和变异体中各扩增出4个位点。对其中2条特异片段进行回收测序,序列同源性为97%。研究认为空间环境条件可以诱导甜椒后代植株基因组DNA发生变异。To study effects of space environment on genomic DNA variation in the offsprings of sweet pepper,the 4th offspring and its ground control were used as materials.The genomic DNA variation was detected by random amplified polymprphic DNA（RAPD）,parts of the different fragments were sequenced.The results showed that the genomic DNA of SP4 after space mutation changed compared to the ground control.Eight polymorphic bands were detected.Four polymorphic bands were amplified in the ground control and variant separately.Two special fragments were sequenced and sequence homology was 97% .So space environment could induce the changes of the genomic DNA in the offsprings of sweet pepper.

关 键 词：甜椒 空间环境 DNA变异 

分 类 号：S641.3[农业科学—蔬菜学]