新疆棉田土壤固氮菌遗传多样性分析  

Genetic Diversity Analysis of Nitrogen-fixing Bacteria in Cotton Soil of the South Xinjiang

作　　者：郑贺云[1] 冯姝[1] 李超[1] 赵夏博[1] 龚明福[1] 

机构地区：[1]新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室塔里木大学生命科学学院,新疆阿拉尔843300

出　　处：《微生物学通报》2010年第4期503-507,共5页Microbiology China

基　　金：国家973计划前期研究专项项目(No.2007CB116303);新疆生产建设兵团基础研究项目(No.2007JC06);新疆生产建设兵团科技攻关计划项目(No.2006GG26)

摘　　要：利用ERIC-PCR和16SrDNA全序列测定方法,研究了新疆棉田土壤中分离获得的58株固氮菌的遗传多样性及系统发育。采用平均连锁法(UPGMA)分析ERIC-PCR的聚类结果表明在Watson距离为0.65左右时可以将供试菌株分为9个大群。选取ERIC-PCR各群中代表菌株进行16SrRNA全序列测定分析,结果表明这些菌株分别属于Enterobacter、Bacillus、Acinetobacter、Pseudomonas、Serratia和Yersinia6个属。To research the effect of nitrogen-fixing bacteria on grow of cotton, we used the technique of ERIC-PCR and 16S rDNA sequencing analysis to study genetic diversity of 58 nitrogen-fixing bacteria isolated from Tarim cotton soils in Xinjiang province. Cluster analysis was used for ERIC-PCR results of these strains by UPGMA. The results showed that the tested strains formed 9 clusters when the Waston distance was about 0.65. The typic strains of 9 clusters were identified as Enterobacter, Bacillus, Acinetobacter, Pseudomonas, Serratia, Yersinia by 16S rRNA sequencing analysis.

关 键 词：连作棉田 固氮菌 ERIC-PCR 新疆 遗传多样性 

分 类 号：S562[农业科学—作物学]