骨折愈合过程中转化生长因子β_1表达的实验研究  

The experimental research of transforming growth factor β 1 expression during fracture healing

作　　者：姬洪全[1] 党耕町[1] 马庆军[1] 王志国[1] 

机构地区：[1]北京医科大学第三医院骨科

出　　处：《中华外科杂志》1998年第2期72-73,I012,共3页Chinese Journal of Surgery

摘　　要：目的观察转化生长因子－β１（ＴＧＦ－β１）在骨折局部的产生和表达量的变化，了解并探讨ＴＧＦ－β１对骨折愈合的调节作用。方法用免疫组织化学方法检测大鼠肋骨骨折愈合不同时期ＴＧＦ－β１的表达。结果在急性损伤反应期，血肿延伸范围内的骨膜内层细胞有少量表达；在膜内成骨期，成骨细胞表达量较高，骨膜细胞表达量少；在软骨形成期，成熟软骨细胞表达量最高；在软骨内成骨期，肥大软骨细胞不表达，成骨细胞表达量较高。结论ＴＧＦ－β１表达量和组织学分布的变化与骨折愈合过程中相应的组织学变化密切相关，而且与相应细胞的功能状态相关，说明ＴＧＦ－β１是调节骨折愈合过程的重要因子。Objective To observe the effect of TGF β 1 in the regulation of fracture healing.Method The expression of transforming growth factor β 1(TGF β 1)in different period of fracture healing was investigated by immunohistochemitry.Results It was found that the expression of TGF β 1 changed in different period.The cells in the cambial layer of the periostlum showed low or negative signal in the immediate injury response period. The osteoblasts differentiated from the periosteum cells stained strongly in the intramembranous ossification period, and the differentiated chondrocytes stained most strongly in the chondrogenesis period. The hypertrophic chondrocytes showed negative signal and the osteoblasts stained strongly in the endochondral ossification period.These results suggested that the expression of TGF β 1 was closely related to the proliferation and differentiation state of repair cells.Conclusion TGF β 1 is intimately involved in the control of fracture healing.

关 键 词：转化生长因子Β 骨折 愈合 基因表达 动物实验 

分 类 号：R683.02[医药卫生—骨科学]