Heymann肾炎致病性抗原Gp330的提取及其致病性研究  

The extraction of the nephritogenic Heymann antigenGp330 and the experimental study on its pathogenicity

作　　者：王钰琦[1,2] 张建民[1,2] 徐衡 

机构地区：[1]解放军总医院胸外科 [2]南京铁道医学院基础部

出　　处：《临床与实验病理学杂志》1998年第2期171-173,I026,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的：制备Ｈｅｙｍａｎｎ肾炎精细致病性抗原Ｇｐ３３０并复制人类膜性肾小球肾炎的动物模型。方法：用超速离心和钙沉降方法获取ＳＤ大鼠刷状缘微绒毛。用ＬＣＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲合层析方法分离纯化Ｈｅｙｍａｎｎ抗原。用多点免疫方法获得抗体并免疫ＳＤ大鼠复制Ｈｅｙｍａｎｎ肾炎模型。结果：获取了Ｈｅｙｍａｎｎ肾炎的精细抗原Ｇｐ３３０并成功地用其抗体免疫ＳＤ大鼠，使其肾小球基底膜有免疫复合物沉积并产生大量蛋白尿。结论：Ｇｐ３３０是一种复制人类膜性肾小球肾炎模型的新抗原。Purpose To extract nephritogenic Heymann antigen Gp330 and resemble animal model of human membranous nephropathy. Methods By using Ca2+ aggregation of contaminant and differential contrifugation methods, to purfiy the brush border membrance from normal renal proximal tubules of the rats. The nephritogenic Heymann antigen Gp330 was isolated and purified from the extraction by means of LCASepharose 4B affinity chromatography. The rats were immunized with antiGp330 antibody. Results The passive Heymann nephritis which had proteinuria and immune deposits was successfully induced in male SD rats. Conclusions Gp330 is a new antigen which may resemble animal model of human membranous nephropathy.

关 键 词：肾小球肾炎 自身抗原 亲和层析 Gp330 

分 类 号：R692.31[医药卫生—泌尿科学]