快速检测西尼罗病毒胶体金免疫层析试纸条方法的建立  

Establishment of the Rapid Detection of West Nile Virus with Gold-immunochromatography

作　　者：王彦芳[1,2] 姜宝芹[2] 邢福彬[2] 杨鹏飞[2] 张丽萍[2] 平芮巾[2] 咸庆杰[1,2] 胡孔新[2] 岳启安[1] 

机构地区：[1]潍坊医学院,山东潍坊261053 [2]中国检验检疫科学研究院

出　　处：《检验检疫科学》2010年第2期10-13,共4页Inspection and Quarantine Science

基　　金：国家公益性行业科研专项(200810162);国家质检总局科研项目(2007IK211;2008IK210)

摘　　要：[目的]建立一种能快速区分、简便诊断西尼罗病毒感染的胶体金免疫层析试纸条方法。[方法]采用柠檬酸三钠还原法制备胶体金颗粒,标记西尼罗病毒NS1蛋白,采用双抗原夹心法制备胶体金免疫层析试纸条,对试纸条进行特异性和敏感性评价。[结果]该试纸条可在15min之内完成检测;在敏感性上,该试纸条对阳性血清的检测较美国Focus公司商品化的ELISA试剂盒检测低1个滴度;对89份阴性血清进行检测,试纸条检出9份阳性,ELISA试剂盒检出3份阳性,两种检测的符合率为86.52%,特异性为89.89%。[结论]初步建立了一种可以用于现场快速检测西尼罗病毒的胶体金免疫层析方法,为进一步大规模应用奠定了基础。It＇s to establish a rapid and convenient gold-immunochromatography for detecting of West Nile Virus.Synthesize 25nm colloidal gold particles by trisodium citrate disoxidation method.Based on the double antigen sandwich technique,NS1 protein of West Nile Virus was coupled with colloidal gold to prepare colloidal probe which was used to detect Anti-NS1 antibody of West Nile Virus.The sensitivity and specificity were evaluated.Testing results can be obtained in 15 minutes with sensitivity of the established method is lower than Focus West Nile Virus IgG ELISA a titre;eighty-nine blood serum samples were tested by means of gold-immunochromatography and ELISA,nine samples were positive tested by gold-immunochromatography,while three samples were positive tested by ELISA,rate of coincidence is 86.52%,when compared with that of ELISA.The gold-immunochromatography is a simple and rapid assay for detecting West Nile Virus on spot which can be further studied to be assembled into commercial kit.

关 键 词：西尼罗病毒 胶体金试纸条 免疫层析法 

分 类 号：R181.2[医药卫生—流行病学]