1,25双羟维生素D_3对成骨样细胞骨架和基因表达的影响  

EFFECT OF 1,25 DIHYDROXYVITAMIN D 3 ON CYTOSKELTAL STRUCTURE AND GENE EXPRESSION IN OSTEOBLAST LIKE CELLS

作　　者：薛延[1,2,3] 袁润英[1,2,3] 相东 王红霞[1,2,3] 李希斌 刘正梅[1,2,3] 吴克斌 范慕贞[1,2,3] 

机构地区：[1]北京积水潭医院生化研究室 [2]北京市神经外科研究所细胞生物学研究室 [3]中日友好医院临床医学研究所生化研究室

出　　处：《解剖学报》1998年第1期67-71,I012,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金

摘　　要：用荧光免疫法研究了１，２５双羟基维生素Ｄ３对大鼠成骨样细胞（ＲＯＳ１７／２．８）细胞骨架的影响和用斑点杂交技术研究了１，２５（ＯＨ）２Ｄ３对人成骨样细胞（ＯＳ－７３２）基因表达的影响。结果表明：在１０－７ｍｏｌ／Ｌ１，２５（ＯＨ）２Ｄ３连续作用４ｄ后，大鼠ＲＯＳ１７／２．８细胞的微管蛋白和波形纤维蛋白明显改善，纤粘蛋白荧光明显增强。而在１，２５（ＯＨ）２Ｄ３作用前后ＯＳ－７３２细胞ｃ－ｍｙｃ和ｍｅｔＤ基因均处于高度活跃表达状态。本文还讨论了细胞分化与细胞骨架及基因表达的关系。The study was carried out to investigate the effects of 1,25 dihydroxyvitamin D 3 1, 25(OH) 2D 3 on cytoskeleton in rat osteoblast like cells (ROS 17/2.8) by immunofluorescent staining methods and on gene expression in human osteoblast like cells (OS 732) by a dot blot techique. The results showed that the microtubules and vimentins improved and the immunoflurorescence of fibronectins increased in ROS 17/2.8 cells after 4 days using 10 -7 mol/L 1,25 (OH) 2D 3 treatment. However c myc and met D highly expressed in OS 732 cells after and before 1,25(OH) 2D 3 treatment. Also the relationships of cell differentiation and cytoskeletal structure and gene expression were discussed.

关 键 词：双羟基维生素D3 成骨样细胞 细胞骨架 基因表达 

分 类 号：R392－33[医药卫生—免疫学] R977.24[医药卫生—基础医学]