煤矿区城市可吸入颗粒物基于DNA损伤的毒理学  

Toxicology of airborne particulate matter in coal mine city results from plasmid DNA assay

作　　者：宋晓焱[1] 邵龙义[1] 周林[1] 张涛[1] 

机构地区：[1]中国矿业大学(北京)地球科学与测绘工程学院,煤炭资源与安全开采国家重点实验室,北京100083

出　　处：《煤炭学报》2010年第4期650-654,共5页Journal of China Coal Society

基　　金：教育部科技创新工程重大项目培育资金资助项目(705022);高等学校博士学科点专项科研基金资助项目(20070290007)

摘　　要：采集2008年煤矿区城市平顶山PM10样品,使用质粒DNA评价法(PlasmidDNAassay)研究了夏季和冬季PM10的毒性。结果表明,不同时间和地点采集的PM10对质粒DNA的损伤差别很大,它们的TD50值(引起50%DNA损伤所需要的颗粒物剂量,以μg/mL表示)相差近10倍;单位质量颗粒物对DNA损伤率顺序:市区冬季>郊区冬季>背景点夏季>郊区夏季>市区夏季;同季节市区和郊区PM10对质粒DNA损伤率差别不大,但不同季节的PM10对质粒DNA损伤率则相差较大,且冬季PM10损伤明显大于夏季;冬季PM10对DNA损伤较大的原因可能与冬季燃煤空气中重金属元素增加有关。通过对颗粒物全样和水溶部分DNA损伤的评价得出,夏季市区和郊区PM10的氧化性损伤能力与颗粒物中的水溶和不溶部分都有较大关系,冬季市区、郊区和夏季背景点PM10的氧化性损伤能力主要来源于颗粒物中的水溶组分。Plasmid DNA assay was employed to investigate toxicity of the PM10 （inhalable particles） collected in the Pingdingshan coal mine city during summer and winter,2008.It is found that the TD50 values （toxic dose of PM10 causing 50% of plasmid DNA damage） of the PM10 collected in different locations and different seasons vary greatly,with the highest value being 10 times of the lowest.The damage rate causes by per unit mass PM10 is higher in the suburban site in winter,follow by the suburban site in winter,comparison site in summer,urban site in summer,and urban site in summer,in descending order.Little difference exists between the urban and suburb sites during a same season,in contrast,there is an obvious variation in the DNA damage rates between the winter and summer,with the winter PM10 having a higher toxicity.The higher toxicity of the winter PM10 may be correlated with the higher contents of the heavy metals during heating by coal-burning.The whole particle solution and soluble fraction of the PM10 were simultaneously tested with the plasmid assay,and the results show that toxicity or oxidative damage to DNA of the PM10 can be sourced from both water-soluble and water-insoluble fractions in the summer urban and suburb samples,whereas the oxidative damage to DNA by the winter urban and suburb samples as well as the summer comparison site samples mainly comes from the water-soluble fraction.

关 键 词：可吸入颗粒物 质粒DNA评价法 氧化性损伤 

分 类 号：X513[环境科学与工程—环境工程]