脂质体包裹氯膦酸二钠剔除大鼠肝脏枯否细胞作用的研究  

Liposome-encapsuled clodronate depletes rat liver Kupffer cells

作　　者：项帅[1] 陈孝平[1] 张伟[1] 梁慧芳[1] 张斌豪[1] 刘旭[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肝脏外科中心,武汉430030

出　　处：《腹部外科》2010年第2期111-113,共3页Journal of Abdominal Surgery

基　　金：国家自然科学基金(30872499)

摘　　要：目的探讨脂质体包裹氯膦酸二钠剔除大鼠肝脏枯否细胞的作用。方法实验组大鼠给予脂质体包裹的氯膦酸二钠,对照组给予等量生理盐水。给药后不同时间点计数ED1、ED2阳性细胞数目;尾静脉注射印度墨汁判断枯否细胞吞噬碳素颗粒的情况;RT-PCR检测枯否细胞受体mRNA的表达情况。结果给药后2d大鼠肝脏吞噬碳素颗粒的枯否细胞消失,ED1、ED2阳性细胞基本消失,PCR检测不到肝脏枯否细胞受体mRNA的表达。给药后第8天ED1阳性细胞开始明显增多,至第11天其数目基本恢复正常。ED2阳性细胞至第11天开始增加,但平均每中倍视野仍仅有(4.8±1.7)个细胞,明显少于对照组的(17.7±2.1)个细胞,差异具有统计学意义(P<0.01)。结论静脉单次注射脂质体包裹氯膦酸二钠至少在1周内能有效剔除肝脏枯否细胞。Objective To assess the role of liposome-encapsuled clodronate（Lip-Clod）depleting rat liver Kupffer cells（KC）. Methods Lip-Clod was administered at a dosage of 0. 1 ml/10 g body weight intravenously. Control rats received the same volume of 0.9% NaCl. Rat livers were harvested at several time points after the injection. Immunohistochemistry for ED1 and ED2 was performed and positive cells were counted to assess the changes of liver KC number. RT-PCR was performed to detect mRNA level of KC receptor. To assess phagocytosis,rats administered with or without Lip Clod were injected with Indian ink and sacrificed 30 rain later. Results Two days after treatment of Lip-Clod, ED1 and ED2 positive cells were nearly absolutely disappeared. Meanwhile, the expression of KCR mRNA could not be detected, and the dense carbon particle uptake was absent after Lip-Clod injection. Eight days after treatment, number of ED1 positive cells was significantly increased and returned to normal level at the day 1 1. A small number of ED2 positive cells could be observed 11 days after treatment. There were only（4. 8 ±1.7）cells in KC depleted group compared to（17.7±2.1 ）in control group （P〈0.01 ）. Conclusion Intravenous administration of a single dose of Lip-Clod can efficiently deplete liver KC at least in one week.

关 键 词：枯否细胞 脂质体 氯膦酸 

分 类 号：R977.2[医药卫生—药品]