骨髓细胞体外成骨能力的实验研究  

Experiment of Bone Forming Ability of Bone Marrow Cell in Vitro

作　　者：陈富林[1] 毛天球[1] 马秦[1] 戴毅敏[1] 韩亮[1] 

机构地区：[1]第四军医大学口腔医学院颌面外科学教研室

出　　处：《华西口腔医学杂志》1998年第3期207-208,I010,共3页West China Journal of Stomatology

摘　　要：通过体外矿化条件观察培养兔骨髓细胞的成骨能力，结果显示：①第３代骨髓细胞碱性磷酸酶染色，７％～８％阳性；②倒置显微镜下观察，细胞传代５～６ｄ后，长满培养瓶的底部，约１０ｄ开始叠层生长，为局灶状，中央出现较多颗粒样改变，１４～１５ｄ时，培养瓶底部肉眼可见白色小结节形成，并逐渐增大；③细胞连续培养３０ｄ，Ｖｏｎ－Ｋａｏｓａ染色显示有局灶状钙盐沉积；④ＳＥＭ观察，细胞呈叠层生长，形成粗大的胶原纤维束，上有钙盐沉淀，３０ｄ时，形成骨小梁样结构。上述结果表明。Bone forming ability of cultured bone marrow cells under mineralized condition in vitro was observed. The results showed that 7%-8% bone marrow cells of the third passage were alkaline phosphate positive. Obseverd by inverted microscope, cells occupied the whole bottom of cell culture bottle in about 5-6 days and began to grow with the manner of bilayers after 10 days, and on the 14 th and 15 th days, small white nodules could be seen on the bottom of bottle. When cultured up to 30 days, Von Kossa staining showed calcium deposition in nodules. Observed by scaning electronic microscope, collagen fibers formed, on which mineral deposited and the trabecular like bone structure formed at the 30 th day. The results suggest that the bone forming ability of cultured bone marrow cells under mineral condition in vitro can be used in tissue engineering.

关 键 词：骨髓细胞 细胞培养 成骨能力 体外培养 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学] R683[医药卫生—基础医学]