大鼠白色念珠菌肺炎与淋巴细胞趋化因子的相关性研究  

Research of correlation between lymphotactin and pulmonary candidiasis albicans in rats

作　　者：贾民勇[1] 山小薪[1] 王文军[1] 湛晓勤[1] 

机构地区：[1]泸州医学院附属医院呼吸内科,646000

出　　处：《国际呼吸杂志》2010年第8期458-461,共4页International Journal of Respiration

摘　　要：目的探讨淋巴细胞趋化因子（lymphotactin，Ltn）与大鼠白色念珠菌肺炎发生发展过程的相关性。方法选用清洁级健康雄性SD大鼠（150～180g，鼠龄30～40d）80只，随机分为模型组与对照组，每组40只。实验组从气管注入白色念珠菌菌液制备白色念珠菌肺炎模型，分别在接种完成后1d、3d、7d、11d处死大鼠（各组10只），取心室血，采用双抗体夹心ABC-酶联免疫吸附试验法测定血清Ltn、γ干扰素（IFN-7）、白介素2（Ib2）水平，取肺组织行组织病理检查及病原体培养计数，应用SPSS13．0统计软件对数据结果进行处理。结果模型1d、3d、7d、11d组均见明显炎症反应，Szapiel评分分别为（1．70±0．48）分、（3．60±0．70）分、（2．90±0．57）分、（1．30±0．52）分，菌体负荷数分别为（3．50±1．07）×10^6cfu／ml、（4．60±1．08）×10^5cfu／ml、（1．30±0．48）×10^5cfu／ml、（0．10±0．32）×10^6du／ml。模型1d、3d、7d组Ltn、IFN-γ、IL-2水平均高于对照组（P〈0．05），模型组Ltn的消长与炎性程度、菌落数、IFN-γ、IL-2水平呈正相关（7=0．816、0．647、0．846、0．823，P值均〈0．05）。结论Ltn参与了真菌感染过程，与IFN-γ、IL-2在消除病原体方面起着相互协同的积极作用。Objective To investigate the correlation between lymphotactin （Ltn） and pulmonary candidiasis albicans in rats. Methods Eighty male SD rats （150-180 g,30-40 days of age） were randomly divided into control group and model group （ n = 40）. In model group, pulmonary candidiasis albieans model was prepared by the injection of Candida albicans from the trachea. Experimental animals were sacrificed at 1 d, 3 d, 7 d, 11 d after vaccination, and ventricle blood was taken. The levels of Ltn, interferon-γ （IFN-γ）,interleukin-2 （IL 2） in serum were measured by double-antibody sandwich ABC- enzyme linked immunosorbent assay. Lung tissues were taken for tissue pathology examination and pathogen count training. The data were analyzed by SPSS 13.0. Results Inflammatory reaction was observed in model 1 d,3 d,7 d, 11 d groups. Szapiel scores were 1.70±0.48,3.60± 0.70,2.90±0.57, 1.304±0.52, respectively. The numbers of bacterial load were （3.50±1.07）×10^6 cfu/ml, （4. 60±1. 08）×10^6 cfu/ml,（1. 30±0.48）×10^6 efu/ml, （0.10± 0.32）×10^6 cfu/ml, respectively. Compared with control group, the levels of Ltn, IFN-γ and IL-2 increased in model 1 d, 3 d, 7 d, 11 d groups （ P 〈 0.05）. There was positive correlation between Ltn level and the degree of inflammation, the number of coenobium,the levels of IFN-γ and IL-2 in model group （ r =0. 816,0. 647,0. 846,0. 823, all P 〈0.05）. Conclusions Ltn might participate in the course of fungal infection, and play a positively synergistieal role with IFN-γ, IL-2 on the elimination of pathogens.

关 键 词：白色念珠菌 肺炎 淋巴细胞趋化因子 

分 类 号：R563.1[医药卫生—呼吸系统]