可降解多孔β-TCP/rhBMP-2人工骨的骨诱导活性研究  

Study on Osteoinductive Potential of Degradable and Porous β-TCP/rhBMP-2 Composite as a Bone Substitute

作　　者：王丹[1,2] 胡蕴玉[1,2] 郑昌琼 谢克难[1,2] 赵广跃 吕荣[1,2] 杨光[1,2] 

机构地区：[1]第四军医大学西京医院全军创伤骨科研究所 [2]四川联合大学高新技术研究院无机材料系

出　　处：《中华骨科杂志》1998年第11期689-691,I003,共4页Chinese Journal of Orthopaedics

摘　　要：目的：检测可降解多孔β－ＴＣＰ／ｒｈＢＭＰ－２人工骨的诱导成骨能力。方法：自制可降解多孔β－ＴＣＰ与ｒｈＢＭＰ－２复合后植入４５只小鼠股后肌袋作为实验组，单纯β－ＴＣＰ植入对侧作对照。术后２４、７２小时、１、２、４、８周分别取材做组织学检查；２、４周取材的部分标本做扫描电镜观察；１、２、４周取材的部分标本做碱性磷酸酶（ＡＬＰ）活性测定。结果：实验组有大量新生软骨和骨形成，随着时间的推移，生成量逐渐增多。对照组无软骨和骨生成。此外，实验组ＡＬＰ活性高于对照组（Ｐ＜０．０１），并且随时间推移而升高（Ｐ＜０．０５）。结论：可降解多孔β－ＴＣＰ／ｒｈＢＭＰ－２具有良好的骨诱导作用。Objective: To examine the osteoinductive potential of degradable and porous β-TCP/rhBMP-2 composite material. Methods: The β-TCP/rhBMP- 2 composite was obtained by combining degradable and porous β-Tricalcium phosphate (β-TCP) that was developed by the authors with recombinant human morphogenetic protein-2 (rhBMP-2) and was implanted into the muscle pouches in the thigh of mice. β-TCP alone was implanted on the opposite site as controls. After the surgery histologic studies (24 and 72 hours,1,2,4 and 8 weeks), scanning electron microscopy(2 and 4 weeks)and alkaline phosphatase assay (1, 2 and 4 weeks) were performed after implantation. Results: There was a large amount of new cartilage and bone formation within the composite material increasing with time. However,there was no new bone formation where β-TCP alone was implanted. The levels of alkaline phosphatase in the β-TCP/rhBMP-2 implants also were increasing with time and were higher than those in controls( P <0.01). Conclusion: β-TCP/rhBMP-2 possesses osteoinductive potential.

关 键 词：磷酸钙类 骨生成 人工骨 骨诱导 β-TCP/BMP 

分 类 号：R687.34[医药卫生—骨科学] R392.4[医药卫生—外科学]