珠江水系特有卷口鱼遗传变异的线粒体Cytb基因序列分析  

Genetic variation of mitochondrial cytochrome b sequences in Ptychidio jordani populations endemic to the Pearl River System

作　　者：范凤娟[1] 章群[1] 赵爽[1] 李名立[1] 周佳怡[1] 余帆洋 唐优良[1] 

机构地区：[1]暨南大学水生生物研究所/广东省水体富营养化与赤潮防治重点实验室,广东广州510632

出　　处：《广东农业科学》2010年第4期161-164,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(30770415);留学归国人员科研启动基金(2005)资助项目

摘　　要：测定了广西合山、柳州、桂平和广东郁南等4个地理群体43尾卷口鱼线粒体Cytb基因1041bp,结果检测到8个单倍型,7个多态位点。4个地理群体的单倍型多样性为0.248～0.700,核苷酸多样性为0.00022～0.00070,表明所分析的卷口鱼群体单倍型多样性和核苷酸多样性低。在分子系统树上,不同地理来源的卷口鱼混杂分布在一起,没有形成明显的谱系结构和地理聚群;4个群体两两间的Fst值为-0.028～0.116,但均不显著(P>0.08);AMOVA分析表明1.70%(P=0.21)的分子差异来自群体间,而98.3%的分子差异位于群体内部;表明4个地理群体间没有显著遗传分化。单倍型网络图呈星状结构,中性检测Tajima’sD和Fu’sFS均为显著负值,核苷酸不对称分布分析呈单峰模式,表明珠江卷口鱼可能曾经历过种群的快速扩张,推测在晚更新世(1.5～3.9万年前)出现种群扩张。1 041 bp sequence of mitochondrial cytochrome b gene of 43 individuals representing 4 populations from Heshan,Liuzhou and Gulping in Guangxi Province and Yunan in Guangdong Province in the Pearl River （PR） system were sequenced,and 8 hapohypes and 7 variable sites were detected. Haplotype diversity ranged from 0.248 to 0.700 and nucleotide diversity ranged from 0.00022 to 0.00070, suggesting that haplotype diversity and nucleotide diversity were both poor in the studied populations. In the Neighbor-Joining tree individuals from different locality were inter/wined without obvious genealogical nor geographic clusters; Pair-wise Fst ranged from -0.028 to 0.116, but not significant （P〉0.08）,AMOVA analysis suggested that geuetie variation within and among populations were 98.3% and 1.70 respectively,indicating that there were no significant genetic divergence among 4 populations. As expected from the star-like networks,the Tajima＇s D tests and Fu＇s FS tests of the whole populations were negative and significant,and the mismatch distributions were unimodal,indicating a late Pleistocene population expansion at about 15-39 kaBP.

关 键 词：卷口鱼 细胞色素B基因 遗传变异 珠江水系 

分 类 号：Q173[生物学—水生生物学]