亚麻与红麻的原生质体融合  

Protoplast fusion of Linum usitatissimum L. and Hibiscus cannabius L.

作　　者：王丽爽[1] 易继财[2] 张宗申[1] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034 [2]华南农业大学生命科学学院,广东广州510642

出　　处：《广东农业科学》2010年第4期191-193,共3页Guangdong Agricultural Sciences

基　　金：辽宁省教育厅科学技术研究重点实验室项目(2008S018)

摘　　要：将亚麻与红麻的愈伤组织在水解酶的作用下分离出原生质体,然后利用PEG法使这2种原生质体融合。结果表明,用2%纤维素酶+1%果胶酶+0.55mol/L甘露醇处理材料,酶解6h后,可以获得大量游离的、圆球形的原生质体;利用40%PEG6000及0.3mol/LCaCl2溶液(含9%甘露醇,pH9.5)将这2种原生质体融合,可以获得稳定的杂合细胞。With the method of enzymolysis, the protoplasts were released from the callus of Linum usitatissimum L. and Hibiscus cannabius L., respectively, and then they were fused by PEG as fusogen. The results showed that spherical protoplasts were available in sufficient quantities by using 2% cellulase ＋ 1% pectinase ＋ 0.5 mol/L mannitol to treat the callus for 6 h. Furthermore, the two different protoplasts could be fused into heterozygous protoplasts using 40% PEG fusion liquid and 0.3 mol/LCaCl： solution （containing 9%mannitol, pH value 9.5）, and these heterozygous protoplasts were stable.

关 键 词：亚麻 红麻 原生质体融合 

分 类 号：Q321.3[生物学—遗传学]