液相色谱串联四极杆质谱检测猪肝猪肉中的齐帕特罗、马布特罗、班布特罗、马贲特罗  

Determination of zilpaterol,mabuterol,bambuterol and mapenterol in liver of pig and pork with HPLC-MS/MS

作　　者：聂建荣[1] 朱铭立[1] 连槿[1] 洪振涛[1] 邓香连[1] 

机构地区：[1]广州市农业标准与监测中心,广东广州510315

出　　处：《广东农业科学》2010年第4期257-259,268,共4页Guangdong Agricultural Sciences

摘　　要：建立了以液相色谱串联四极杆质谱(HPLC-MS/MS)检测猪肝猪肉中齐帕特罗、马布特罗、班布特罗、马贲特罗的分析方法。样品经MCX柱净化浓缩后,用C18液相色谱柱分离,通过电喷雾(ESI)离子源电离,多反应监测(MRM)作定性和定量分析。在0.25～50μg/L的范围内线性良好(r>0.999)。在0.5、4.0、10μg/kg添加水平下,回收率为70.5%～91.9%,相对标准偏差为3.7%～7.4%。方法检测限(S/N=5)可达0.5μg/kg。The HPLC-MS/MS method was presented to analyze zilpaterol, mabuterol, bambuterol and mapenterol in liver of pig and pork. The sample was purified and concentrated by MCX column, chromatographic conditions included a C18 column. Qualitative and quantitative analysis which ionized by electrospray ionization interface（ESI） were carried on through MRM mode. There was a good linear correlation in the range of 0.25-50 μg/L （r〉0.999）.The recoveries ranged from 70.5% to 91.9% At the add concentration level of 0.5, 4.0,10 μg/kg, the RSD were between 3.7% and 7.4%.hs limit of detection reached 0.5 μg/kg.

关 键 词：HPLC—MS/MS 检测 齐帕特罗 马布特罗 班布特罗 马贲特罗 

分 类 号：O657.63[理学—分析化学]