骨折愈合过程中转化生长因子-β_1基因表达  

The Gene Expression of Transforming Growth Factor-β 1 in the Process of Fracture Healing

作　　者：姬洪全[1] 党耕町[1] 马庆军[1] 王志国[1] 

机构地区：[1]北京医科大学第三医院骨科

出　　处：《中华骨科杂志》1998年第9期544-546,I003,共4页Chinese Journal of Orthopaedics

摘　　要：目的：研究转化生长因子－β１基因在骨折后不同修复细胞中的表达及其变化，了解其在骨折愈合过程中的来源及产生机理。材料与方法：大鼠肋骨骨折标本，制作脱钙石蜡切片，用原位杂交技术检测骨折修复细胞中的转化生长因子－β１信使核糖核酸。结果：骨折后２、５、１０、１５天分别在相应修复细胞中检测到转化生长因子－β１ｍＲＮＡ的阳性信号，即骨膜生发层细胞、膜内成骨区的成骨细胞、软骨形成区的软骨细胞及软骨内成骨区的成骨细胞。其中成熟软骨细胞内信号最强，而肥大软骨细胞内显示阴性信号。结论：转化生长因子－β１可以在骨折局部由修复细胞自身产生，其产生量与细胞的分化状态、功能水平有关，它可能以自分泌或旁分泌方式参与骨折愈合的调节。Objective: The gene expression of TGF-β 1 in differentre pair cells of fracture healing was studied to investigate the mechanism of production of TGF-β 1 in the repair area. Methods: The TGF-β 1 mRNA in paraffin sections of rat rib fracture specimens was detected by in situ hybridization. Results: The TGF-β 1 mRNA can be detected in different repair cells i.e. the cambial layer cells of periosteum near the fracture line , the osteoblasts in the intramembranous ossification region, the chondrocytes in the chondrogenesis region and the osteoblasts in the endochondral ossification region. The mature chondrocytes stain most strongly among all of the repair cells. The hypertrophic chondrocytes show negativesignal. Conclusion: The gene transcription of TGF-β 1 occurred in different repair cells. So TGF-β 1 can be produced locally by the repair cells. It may play its role through an autocrine or paracrinepathway.

关 键 词：骨折愈合 原位杂交 转化生长因子Β 

分 类 号：R683.02[医药卫生—骨科学]