EB病毒BHRF_1基因聚合酶链反应扩增及其鉴定  

Amplification and identification of EBV BHRF 1 gene

作　　者：戴克胜[1,2] 朱振宇[1,2] 马涧泉[1,2] 

机构地区：[1]蚌埠医学院临床血液学教研室 [2]广州中山医科大学生物化学教研室

出　　处：《蚌埠医学院学报》1998年第5期298-299,F002,共3页Journal of Bengbu Medical College

基　　金：国家教育部博士点基金

摘　　要：目的：ＢＨＲＦ１的研究对于认识ＥＢＶ有关肿瘤和其它疾病的发病机理、寻找预防、治疗途径有十分重要的意义。方法：以Ｂ９５８细胞ＤＮＡ为模板，设计一对引物，用ＰＣＲ方法扩增ＢＨＲＦ１基因，用目的基因内的两个限制性内切酶位点进行酶切鉴定。结果：扩增产物长５９２ｂｐ，与预期结果一致，两个酶所切下的四个片段长度也与理想值相符。结论：本研究首次采用ＰＣＲ方法，成功地获得完整的ＥＢ病毒ＢＨＲＦ１基因，从而为该基因的进一步研究和应用奠定基础。Objective:To study the function of BHRF 1 gene for elucidating the mechanism of EBV related tumours and other diseases,their prevention and treatment.Methods:The BHRF 1 gene was amplified from template DNA of B 95 8 cell by polymerase chain reaction,and was identified with method of digestion with two unique restriction endonucleases.Results:The amplified fragment was about 592 bp in length.The base pairs of these four bands which cleaved with two unique restriction endonucleases are in coincidence with lengthes as predicted.Conclusions:The intact BHRF 1 gene,for the first time,is obtained by using PCR technique and primers designed by ourselves.The study provides a model for further research on EBV BHRF 1 gene.

关 键 词：EB病毒 BHRF1基因 聚合酶链反应 鼻咽肿瘤 

分 类 号：R373.11[医药卫生—病原生物学] R739.63[医药卫生—基础医学]