大鼠脑干面口部运动神经元与5-HT样、SP样、L-ENK样、GABA样和Gly样阳性终末间的突触联系  

SYNAPTIC CONNECTIONS BETWEEN SEROTONIN ,SUBSTAN CEP ,LEU ENKEPHALIN ,GABA ,AND GLYCINE LIKE IMMUNOREACTIVE TERMINALS AND MOTONEURONS IN THE BRAINSTEM OROFACIAL MOTOR NUCLEI OF THE RAT

作　　者：李云庆[1] 王智明[1] 

机构地区：[1]第四军医大学解剖学教研室

出　　处：《解剖学报》1998年第4期351-355,I003,共5页Acta Anatomica Sinica

基　　金：国家杰出青年科学基金

摘　　要：为了观察５－羟色胺（５－ＨＴ）样、Ｐ物质（ＳＰ）样、亮氨酸－脑啡肽（Ｌ－ＥＮＫ）样、γ－氨基丁酸（ＧＡＢＡ）样和甘氨酸（Ｇｌｙ）样阳性终末与脑干面口部运动核（三叉神经运动核、面神经核和舌下神经核）间的突触联系，用ＨＲＰ逆行追踪与免疫组织化学染色相结合的双标技术，在电镜下对５－ＨＴ样、ＳＰ样、Ｌ－ＥＮＫ样、ＧＡＢＡ样和Ｇｌｙ样阳性终末与ＨＲＰ逆标面口部运动神经元间的突触联系进行了观察。５－ＨＴ样、ＳＰ样、Ｌ－ＥＮＫ样、ＧＡＢＡ样和Ｇｌｙ样阳性终末在脑干面口部运动核内呈均匀分布，没有区域特异性。将ＨＲＰ注入面口部肌肉或相应的周围神经后，ＨＲＰ逆标神经元见于脑干运动核不同部位内支配相应肌肉的运动神经元。５－ＨＴ样、ＳＰ样和Ｌ－ＥＮＫ样阳性终末主要与ＨＲＰ逆标运动神经元的树突形成非对称性的轴－树突触；ＧＡＢＡ样和Ｇｌｙ样阳性终末则主要与ＨＲＰ逆标运动神经元的树突形成对称性的轴－树突触；偶见上述阳性轴突终末与ＨＲＰ逆标运动神经元的胞体形成轴－体突触。结果提示：主要来自脑干运动前神经元的５－ＨＴ样。In order to study the synaptic connections between the serotonin(5 HT) ,substance P(SP) ,leucine enkephalin (L ENK) ,γ aminobutyric acid (GABA) ,and glycine (Gly) like immunoreactive (LI) axon terminals and the motoneurons within the brainstem orofacial motor nuclei (trigeminal motor,facial,and hypoglossal nuclei)of the rat,a double labeling method by combination of retrograde tracing with immunohistochemical staining was used in the present study. 5 HT , SP ,L ENK ,GABA ,and Gly LI axon terminals were densely observed in the brainstem orofacial motor nuclei.The distribution patterns of those axon terminals had no topographic differences within the different subregions of the motor nuclei.After injecting horseradish peroxidase(HRP) into the orofacial muscles or related nerves,HRP retrogradely labeled neurons were found in different subregions of the motor nuclei,in which motoneurons send their axons to innervate the orofacial muscles.5 HT ,SP ,and L ENK LI axon terminals made asymmetrical axo dendritic synapses with dendrites of the HRP retrogradely labeled motoneurons,whereas GABA and Gly LI axon terminals formed symmetrical axo dendritic synapses with dendrites of the HRP retrogradely labeled motoneurons.Occasionaly,axo somatic synapses were encountered between 5 HT , SP ,L ENK ,GABA ,and Gly LI axon terminals and HRP retrogradely labeled neuronal cell bodies of the motoneurons.The present results indicate that 5 HT ,SP ,and L ENK LI or GABA and Gly LI axon terminals which originated mainly from brainstem premotor neuron pool might have excitatory or inhibitory effects on orofacial motoneurons in the brainstem orofacial motor nuclei.

关 键 词：突触联系 大脑运动皮质 5-HT SP L-ENK GABA 

分 类 号：R338[医药卫生—人体生理学]