小鼠卵细胞质内显微注入单精子受精的实验研究  

EXPERIMENTAL STUDY ON INTRACYTOPLASMIC SPERM INJECTION IN THE MOUSE EGG

作　　者：杨益寿[1] 熊素芳[1] 龙文 李鸣 夏明珠[1] 汪昌介 

机构地区：[1]湖北医科大学附属第一医院

出　　处：《解剖学报》1998年第4期441-445,I011,共6页Acta Anatomica Sinica

摘　　要：为了探讨提高小鼠卵细胞质内单精子注入受精率的方法，选取鼠龄１２～１４周的健康昆明白小鼠作为精子和卵子的供体，采用ＩＣＳＩ技术，以受精后二细胞卵裂的形成率为指标，了解不同采卵时间、不同微注射针参数及不同培养液对细胞质内单精子注入的影响。结果表明，ｈＣＧ注射后１８～１９ｈ采卵，用针尖内径为４～５μｍ、斜面角度为３５～４０度的微注射针进行ＩＣＳＩ操作，受精后卵子置ＣＺＢ中培养可获得较多的２细胞胚，卵裂率明显高于其余各组（２８．１０％），有高度显著性差异（Ｐ＜０．０１）。结果提示：恰当的采卵时间。To study methods raising average percentage of fertilization by intracytoplasmic sperm injection (ICSI) in muose eggs. Methods: Kunming white mice (12 14 weeks of age) were use as donors of sperms or eggs. The presentation of 2 cell blastomere was considered a success of fertilization. The influence of different parameter of micropipette,phases of collecting eggs and media on ICSI was investigated. Results: The results showed that the average percentage of 2 cell stage was clearly higher (28 10%) than that of other groups while using micropipette tip with internal diameter 4 5 μm and angle of incline plane 35 40, collecting eggs on 18 19 h after injecting human chorionic gonadotrophin、 application of Chatot Ziomek Bavister medium. Conclusion: Results suggest that proper parameter of micropipette,phases of collecting eggs and selected medium were closely related to percentage of fertilization during ICSI course.

关 键 词：卵细胞质 单精子注射 显微注射针 小鼠 受精 

分 类 号：Q132.1[生物学—普通生物学]