穿孔素蛋白及基因在子宫内膜中的表达  

Perforin Protein and Gene Expression in the Different Phases of Endometrium

作　　者：田秀珠[1] 张丽珠[1] 吴燕[1] 张小为[1] 

机构地区：[1]北京医科大学第三临床医学院妇产科

出　　处：《中华妇产科杂志》1998年第10期591-593,I017,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的探讨穿孔素蛋白及基因在不同时期子宫内膜中表达的规律，及其在生殖系统中的作用。方法采用免疫组化与原位杂交技术，观察１０例增殖期、６８例分泌各期、２０例早孕期及４例绝经后子宫内膜中穿孔素蛋白的表达及基因阳性细胞的分布特点、表型特征和数量变化规律。结果在各期子宫内膜中，产生穿孔素蛋白的细胞位于子宫内膜间质，此细胞表型特征为ＣＤ５６＋淋巴细胞（自然杀伤样细胞）。在各期子宫内膜中表达穿孔素蛋白的阳性细胞数量不同，从增殖期、分泌期至早孕期，此阳性细胞数量逐渐增多，各组间差异有显著性（Ｐ＜０．０５），绝经后子宫内膜中无穿孔素蛋白表达。在增殖期，穿孔素基因阳性细胞大多呈局灶状分布，与同期表达穿孔素蛋白的阳性细胞呈少量单细胞不一致分布，分泌期与早孕期子宫内膜中表达穿孔素基因的阳性细胞呈弥散状分布，并且早孕期此阳性细胞数量增多。结论子宫内膜中穿孔素蛋白和基因表达，可能参与月经来潮的调节，利于胚胎着床和妊娠的顺利进行。Objective To study the expression of perforin protein and gene in the different phases of endometrium and the role of perforin in the reproductive system. Methods Immunohistochemical and in situ hybridization techniques were employed to demonstrate the population, phenotype of the perforin positive cell in endometrum of different phases of menstrual cycle, first trimester decidua and postmenopausal endometrium. Results Perforin positive cells were found to be located in the endometrial stroma and expressed natural killer like cell surface marker (CD 56 antigen, also named NK like cell or CD 56 + lymphocyte). The quantitative analysis of perforin protein cells demonstrated an increase in number from proliferative endometrium to secretory endometrium through first trimester decidua ( P <0 05). These cells were absent in the postmenopausal endometrium. Perforin mRNA positive cells formed islands in proliferative endometrium stroma, but the perforin protein expression cells were scattered. There was difference between the perforin gene expression and protein expression in proliferative endometrium. In first trimester decidua, perforin mRNA positive cells increased. Some perforin mRNA positive cells located around spiral arterioles were observed. Conclusions Expression of perforin in the different phases of endometrium is specific and plays an important role in the reproductive function. They probably exert some influence on embryo implantation and may be involved in endometrial stromal breakdown during menstruation.

关 键 词：子宫内膜 基因表达 淋巴细胞 穿孔素蛋白 

分 类 号：R339.22[医药卫生—人体生理学] R392.12[医药卫生—基础医学]