细胞培养物中的逆转录病毒检测  

DETECTION OF RETROVIRUSES IN CELL CULTURES

作　　者：张荣兴[1] 吕沅冈[1] 杨正洪 喻峰 朱德厚 葛锡锐[1] 

机构地区：[1]中国科学院上海细胞生物学研究所,上海200031

出　　处：《细胞生物学杂志》1998年第3期W001-W003,共3页Chinese Journal of Cell Biology

基　　金：中国科学院"八五"重点项目基金

摘　　要：本文用非放射性法测定了K562,SP2/0,L-02,SPC-A-1,SMMC-7721和Hut-102等6个细胞培养液超离心沉淀物逆转录酶的含量,间接证明细胞培养物中逆转录病毒的存在。结果表明,Hut-102细胞为阳性,其它5个细胞系为阴性。By non-radioactive reverse transcriptase (RT)assay ,we detected RT activity of ultracentrifuged peliet of culture supernatant in six different cell lines. The results indicate that only Hut-102 cell shows positive reaction,but K562, SP2/0,L-02,SPC-A-1 and SMMC-7721 cells are negative. We suggest that Hut-102 cells release retrovirus as indicat-ed by Poiesz,B.,et al, It is very important to detect retroviruses in cell cultures because retroviruses are associated with a variety of diseases,including some lethal diseases.

关 键 词：逆转录病毒 逆转录酶 细胞系 

分 类 号：Q939.4[生物学—微生物学]