急性脊髓损伤促甲状腺素释放激素治疗的实验研究  

Experimental Study of Thyrotropin-Releasing Hormone for the Treatment of Acute Spinal Cord Injury

作　　者：朱悦[1] 王星铎[1] 包峰[2] 吕刚[1] 金成壁[1] 

机构地区：[1]中国医科大学第一临床学院骨科,沈阳110001 [2]中国医科大学脑研究所

出　　处：《中华骨科杂志》1998年第12期747-750,I002,共5页Chinese Journal of Orthopaedics

摘　　要：目的:证明促甲状腺素释放激素对实验性脊髓损伤的治疗作用。材料和方法:采用改良Allen法造成Wistar大鼠不完全性脊髓损伤。应用神经学功能评分法、定量酶及免疫组织细胞化学方法和电镜技术进行疗效观察。结果:(1)治疗组神经学功能评分明显高于对照组。(2)损伤后不同时间治疗组前角细胞乙酰胆碱酯酶活性均高于对照组,于损伤后2周其活性基本恢复正常;而酸性磷酸酶活性均低于对照组,于损伤后4周基本恢复正常。损伤后1周治疗组前角细胞尼氏体含量高于对照组。(3)治疗组脊髓白质一定面积内轴突数量多于对照组。(4)超微结构观察显示治疗组神经组织损伤及出血程度均轻于对照组。结论:促甲状腺素释放激素对脊髓损伤的治疗作用不仅表现在促进运动功能恢复方面,而且在组织形态学上也有体现。Purpose: To confirm the therapeutic effects of thyrotropin-releasing hormone(TRH) on the experimental spinal cord injury. Method: Wistar rats were subjected to incomplete spinal cord injury using the modified Allen method. The effects were observed by means of neurologic scoring, quantitative enzyme histocytoehemistry、quantitative immunohistocytochemistry and electron microscopy. Results: 1) The treated group exhibited significantly higher scores than the control group. 2) Acetylcholinesterase (AchE) activity of anterior horn cells in the treated group was higher than that of the control group invarious time, and its activity restored to normal level two weeks after injury; Acid phosphatase(AePase) activity was lower than that of the control group in various time. and its activity restored to normal level four weeks after injury. The content of Nissl's bodies in anterior horn cells in the treated group was higher than that of the control goup one week after injury. 3)The number of axon in a certain area of spinal white matter in the treated group was more than that of the control group. 4)Ultrastructural observation showed that both the nerve tissue injury and the extent of hemorrhage were milder in the treated group than that of the control group. Conclusion: The therapeutic effects of TRH on the spinal cord injury' are shown not only in the improving recovery of motor function, but also in the morphology.

关 键 词：脊髓损伤 受体 促甲状腺素 释放激素 

分 类 号：R651.205[医药卫生—外科学]